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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 6

sp. nov., a denitrifying bacterium, isolated from pond water for No Access

Abstract

A novel Gram-stain-negative strain, designated JM10B15, was isolated from pond water for collected from Jiangmen City, Guangdong province, south PR China. Cells of the strain were aerobic, rod-shaped, and motile by lateral flagella. JM10B15 could grow at 15–40 °C, pH 6.0–9.5, and in 0–3.0 % NaCl, with optimal growth at 25–35 °C, pH 7.5–8.5, and in 0 % NaCl, respectively. Furthermore, this strain grew well on Reasoner's 2A agar but not on nutrient broth agar or Luria–Bertani agar. JM10B15 was a denitrifying bacterium capable of removing nitrites and nitrates, and three key functional genes, , , and , were identified in its genome. The results of phylogenetic analyses based on the 16S rRNA gene and genome sequences indicated that JM10B15 belonged to the genus . JM10B15 showed the highest 16S rRNA sequence similarity to YJ-T1-11 (98.8 %), followed by IFAM 1031 (98.6 %) and HB-1 (98.1 %). The average nucleotide identity and digital DNA–DNA hybridization values between JM10B15 and the other type strains of genus were 78.1–82.1 % and 18.4–22.1 %, respectively. The major fatty acids of strain JM10B15 were summed feature 8 (C 6 and/or C 7) and C 7 11-methyl. In addition, the major respiratory quinone of this novel strain was Q-10, and the predominant polar lipids were phosphatidylglycerol, phosphatidylethanolamine, four unidentified phospholipids, three unidentified lipids, and an unidentified aminophospholipid. Results of analyses of the phylogenetic, genomic, physiological, and biochemical characteristics indicated that JM10B15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JM10B15 (=GDMCC 1.4148=KCTC 8140).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): denitrifying bacterium , Gemmobacter denitrificans , nitrogen removal , novel species and polyphasic taxonomy
Funding
This study was supported by the:
  • Guangdong Strategic Special Fund for Rural Revitalization (Award 2023-WBH-00-001)
    • Principle Award Recipient: HonghuiZhu
  • Guangdong Special Support Program (Award 2021JC06N628)
    • Principle Award Recipient: HonghuiZhu
  • GDAS’ Project of Science and Technology Development (Award 2022GDASZH-2022010101)
    • Principle Award Recipient: MingxiaZhang
  • GDAS’ Project of Science and Technology Development (Award 2022GDASZH-2022010202)
    • Principle Award Recipient: YulianZhang
  • National Natural Science Foundation of China (Award 32200086)
    • Principle Award Recipient: MingxiaZhang
  • National Natural Science Foundation of China (Award 32070115)
    • Principle Award Recipient: MingxiaZhang
© 2024 The Authors
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2024-06-17
2025-05-21
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Gemmobacter denitrificans sp. nov., a denitrifying bacterium, isolated from pond water for Litopenaeus vannamei
Int J Syst Evol Microbiol 74, 006430 (2024); https://doi.org/10.1099/ijsem.0.006430
/content/journal/ijsem/10.1099/ijsem.0.006430
/content/journal/ijsem/10.1099/ijsem.0.006430
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