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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 6

sp. nov., isolated from the Haima cold seep, South China Sea No Access

Abstract

A Gram-stain-negative, orange-yellow, rod-shaped bacterium, designated strain SCSIO 19198, was isolated from sediment of the Haima cold seep in the South China Sea, PR China. The strain was aerobic and non-motile. Growth of strain SCSIO 19198 occurred at pH 7–9 (optimum, pH 7), 15–37 °C (optimum, 25–32 °C) and with 3–8 % (w/v) NaCl (optimum, 3–6 % NaCl). Phylogenetic analyses based on 16S rRNA sequences revealed that strain SCSIO 19198 belonged to the genus , having the highest similarity to HD-3 (98.35 %), followed by KYW589 (95.17 %) and GYP-24 (94.89 %). The DNA G+C content was 35.92 mol%. The average nucleotide identity value between the genome of strain SCSIO 19198 and that of HD-3 was 88.49 %. The digital DNA–DNA hybridization value between strain SCSIO 19198 and HD-3 was 36 %. The major fatty acids (>10 %) of strain SCSIO 19198 were iso-C, iso-C G, summed feature 3 (C 6/C ω7) and anteiso-C. MK-6 was the only detected respiratory quinone. The polar lipids of strain SCSIO 19198 included phosphatidylethanolamine, two aminolipids, glycolipid and two unidentified lipids. The phenotypic, phylogenetic, chemotaxonomic and genomic data clearly suggest that strain SCSIO 19198 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SCSIO 19198 (=MCCC 1K08674=KCTC 102078).

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Keyword(s): cold seep sediment , Hwangdonia lutea sp. nov. and polyphasic taxonomy
Funding
This study was supported by the:
  • Natural Science Foundation of Guangxi Zhuang Autonomous Region (Award 2023GXNSFAA026388)
    • Principle Award Recipient: HongfeiSu
  • Key Science and Technology Project of Hainan Province (Award ZDKJ202018)
    • Principle Award Recipient: JieLi
© 2024 The Authors
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2024-06-17
2025-06-24
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Hwangdonia lutea sp. nov., isolated from the Haima cold seep, South China Sea
Int J Syst Evol Microbiol 74, 006428 (2024); https://doi.org/10.1099/ijsem.0.006428
/content/journal/ijsem/10.1099/ijsem.0.006428
/content/journal/ijsem/10.1099/ijsem.0.006428
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