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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 6

sp. nov. and sp. nov., isolated from artificial pond water and sp. nov., isolated from a root No Access

Abstract

Two novel strains, designated APW6 and APW11, were isolated from artificial pond water, and one novel strain, designated PFR6, was isolated from a root. These strains were found to be Gram-negative, rod-shaped, motile by means of flagella, and oxidase-positive. Growth conditions of the type strains were as follows: APW6, 15–43 °C (optimum, 28 °C), pH 6.0–12.0 (optimum, pH 7.0), with no salinity; APW11, 4–35 °C (optimum, 25 °C), pH 6.0–11.0 (optimum, pH 9.0), with 0–1 % NaCl (w/v, optimum 0 %); PFR6, 10–38 °C (optimum 28 °C), pH 6.0–12.0 (optimum, pH 7.0), with 0–2 % NaCl (w/v; optimum, 0 %). Strains APW6, APW11, and PFR6 belonged to the genus , having the most 16S rRNA gene sequence similarity to DSM 654 (98.1 %), CHU3 (98.7 %), and CCUG 52769 (98.1 %). The estimated genome sizes of APW6, APW11, and PFR6 were 50 50 473, 56 70 008, and 52 16 869 bp, respectively and the G+C contents were 69.5, 66, and 68.5 mol%. The digital DNA–DNA hybridization, average amino acid identity, and average nucleotide identity values among the novel strains and related taxa were all lower than 22.4, 74.7, and 78.9 %, respectively. The predominant cellular fatty acids (>10 %) of all strains were summed feature 3 (comprising C 6 and/or C 7) and C. PFR6 also had summed feature 8 (comprising C  7 and/or C  6) as a major fatty acid. The polar lipid profile of all strains contained phosphatidylethanolamine, phosphoaminoglycolipid, and phosphoglycolipid. The distinct phylogenetic, physiological, and chemotaxonomic features reported in this study indicate that strains APW6, APW11, and PFR6 represent novel species within the genus , for which the names sp. nov., with the type strain APW6 (=KACC 22877=TBRC 16606), sp. nov., with the type strain APW11 (=KACC 22878=TBRC 16607), and sp. nov (=KACC 23257=TBRC 17653) are respectively proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): artificial pond , Comamonadaceae , novel species , root and Roseateles
Funding
This study was supported by the:
  • National Research Foundation of Korea (Award 2022R1F1A1070108)
    • Principle Award Recipient: TaegunSeo
  • National Institute of Biological Resources (Award NIBR202304204)
    • Principle Award Recipient: TaegunSeo
© 2024 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.006426
2024-06-13
2025-05-17
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Roseateles subflavus sp. nov. and Roseateles aquae sp. nov., isolated from artificial pond water and Roseateles violae sp. nov., isolated from a Viola mandshurica root
Int J Syst Evol Microbiol 74, 006426 (2024); https://doi.org/10.1099/ijsem.0.006426
/content/journal/ijsem/10.1099/ijsem.0.006426
/content/journal/ijsem/10.1099/ijsem.0.006426
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