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Abstract

Strain I65 (=KACC 22647=JCM 35315), a novel Gram-stain-negative, strictly aerobic, non-motile, non-spore-forming, rod-shaped, and orange-pigmented bacterium was isolated from influent water of a wastewater treatment system after treatment with several antibiotics, such as meropenem, gentamicin, and macrolide. The newly identified bacterial strain I65 exhibits significant multi-drug and heavy metal resistance characteristics. Strain I65 was grown in Reasoner's 2A medium [0 %–2 % (w/v) NaCl (optimum, 0 %), pH 5.0–10.0 (optimum, pH 7.0), and 20–45°C (optimum, 30 °C)]. Phylogenetic analysis based on 16S rRNA gene sequencing confirmed that strain I65 was closely related to CCBAU 05354 (99.56 % sequence similarity), THG-DN5.5 (97.51 %), and GR10-1 (97.09 %). Further analysis of the whole-genome sequence confirmed that the digital DNA–DNA hybridization, average nucleotide identity, and average amino acid identity values between strain I65 and CCBAU 05354 were 23.4, 80.7, and 85.0 %, respectively, suggesting that strain I65 is distinct from . The genome size of strain I65 was 6.1 Mbp, as assessed using the Oxford Nanopore platform, and its genomic DNA G+C content was 43.0 mol%. The major fatty acids of strain I65 were iso-C and iso-C G, and the major respiratory quinone was MK-7. Moreover, the major polar lipid of strain I65 was phosphatidylethanolamine. Based on genotypic, chemotaxonomic, and phenotype data, strain I65 represents a novel species belonging to the genus , for which the name sp. nov. is proposed. The type strain is I65 (=KACC 22647=JCM 35315).

Funding
This study was supported by the:
  • National Research Foundation of Korea (NRF) Grant (Award NRF-2020M3A9H5104237)
    • Principle Award Recipient: WoojunPark
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2024-06-17
2024-07-15
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