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Abstract

A Gram-stain-positive, strictly anaerobic, endospore-forming and rod-shaped (0.6–0.8×2.7–13.1 µm) bacterium, designated as 5 N-1, was isolated from a yellow water sample collected from the manufacturing process of Nongxiangxing baijiu in the Yibin region of Sichuan, PR China. Growth occurred at 15–40 °C (optimum growth at 37 °C), at pH 6.0–9.0 (optimum growth at pH 7.0) and in NaCl concentrations of 0–1 % (w/v) and ethanol concentrations of 0–2 % (v/v). The major fatty acids in strain 5 N-1 were C, C and C. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unidentified aminophospholipids and one unidentified lipid. Phylogenetic analysis of its 16S rRNA gene sequence indicated that strain 5 N-1 was most closely related to YB-6 (97.70 %) and DSM 12992 (97.56 %). The average nucleotide identity and digital DNA‒DNA hybridization values between strain 5 N-1 and the above two type strains were 80.89 and 80.05 % and 25.80 and 25.30 %, respectively, which were all below the species thresholds. The genome size of strain 5 N-1 was 3.5 Mbp and the DNA G+C content was 27.5 mol%. Based on the results of phenotypic and genotypic analyses, strain 5 N-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 5 N-1 (=CICC 24886=JCM 35355).

Funding
This study was supported by the:
  • National Infrastructure for Microbiological Resources (Award NMRC-2023-4)
    • Principle Award Recipient: SuYao
  • TaiShan Industrial Experts Program (Award tscx202306143)
    • Principle Award Recipient: SuYao
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2024-06-12
2024-07-15
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