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Abstract

During a study on the diversity of culturable actinobacteria from coastal halophytes in Thailand, strain LSe6-5 was isolated from leaves of sea purslane ( L.), and a polyphasic approach was employed to determine its taxonomic position. The 16S rRNA gene sequences analysis indicated that the strain was most closely related to Tu 6233 (99.2 %), YIM M13156 (99.1 %), and PB261 (98.7 %). The genome of strain LSe6-5 was estimated to be 4.33 Mbp in size, with DNA G+C contents of 74.3%. A phylogenomic tree based on whole-genome sequences revealed that strain LSe6-5 formed a clade with DSM 45722, indicating their close relationship. However, the average nucleotide identity (ANI)-, ANI-MUMmer, and dDDH values between strain LSe6-5 with DSM 45722 (87.1, 88.9, and 33.0 %) were below the thresholds of 95–96 % ANI and 70 % dDDH for identifying a novel species. Furthermore, strain LSe6-5 showed morphological and chemotaxonomic characteristics of the genus . Cells were motile, rod-shaped, and Gram-stain-positive. Optimal growth of strain LSe6-5 occurred at 28 °C, pH 7.0, and 0–3 % NaCl. The whole-cell hydrolysates contained -diaminopimelic acid as the diagnostic diamino acid, with galactose, glucose, mannose, and ribose as whole-cell sugars. The predominant menaquinones were MK-9(H) and MK-9(H). The polar lipid profile was composed of diphosphatidylglycerol, hydroxyphosphatidylethanolamine, phosphatidylinositol, glycophosphatidylinositol, an unidentified phospholipid, and an unidentified lipid. Major cellular fatty acids were -C, -C, and -C. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, it is supported that strain LSe6-5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain LSe6-5 (=TBRC 16417= NBRC 115929).

Funding
This study was supported by the:
  • National Research Council of Thailand (NRCT) (Award 186516, N25A660324)
    • Principle Award Recipient: DuangmalKannika
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2024-06-04
2024-06-17
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