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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 6

sp. nov., isolated from garden soil Open Access

Abstract

A novel bacterial strain, designated as MAH-18, was isolated from soil sampled in a flower garden. Cells of strain MAH-18 were Gram-stain-positive, aerobic, motile, and rod-shaped. The colonies were beige in colour, smooth, and spherical when grown on Reasoner's 2A agar medium. Strain MAH-18 grew at 20–40 °C, pH 6.0–8.0, and 0–1.0 % NaCl. Cells were able to hydrolyse aesculin, gelatin, and Tween 20. According to the 16S rRNA gene sequence comparisons, the isolate was determined to be a member of the genus and most closely related to OS4 (97.9 %), DS-30 (97.9 %), GW-9 (97.6 %), mbc-2 (97.5 %), HWE 2-02 (97.4 %), and GBK3QG-3 (96.3 %). Strain MAH-18 has a draft genome size of 4 788 325 bp (eight contigs), 4572 protein-coding genes, 46 tRNA, and three rRNA genes. The average nucleotide identity and digital DNA–DNA hybridization values between strain MAH-18 and the closest type strains were 81.5–83.4 % and 24.4–25.8 %, respectively. genome mining revealed several biosynthetic gene clusters in the genome of the novel strain MAH-18. The G+C content of the genomic DNA of strain was 72.2 mol% and the predominant isoprenoid quinone was MK-8 (H). The main polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, and unknown phospholipids. The major cellular fatty acids were determined to be C iso and C 6. The DNA–DNA hybridization results and phenotypic, genotypic, and chemotaxonomic data demonstrated that strain MAH-18 represents a novel species, for which the name sp. nov. is proposed, with MAH-18 as the type strain (=KACC 19744=CGMCC 1.13656).

  • Received:
  • Accepted:
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Keyword(s): 16S rRNA , genome sequence , Gram-stain-positive and Nocardioides agri
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-06-18
2025-03-23
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Nocardioides agri sp. nov., isolated from garden soil
Int J Syst Evol Microbiol 74, 006407 (2024); https://doi.org/10.1099/ijsem.0.006407
/content/journal/ijsem/10.1099/ijsem.0.006407
/content/journal/ijsem/10.1099/ijsem.0.006407
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