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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 5

sp. nov., a novel endophytic actinomycete isolated from the leaves of No Access

Abstract

Strain HUAS 3-15 was isolated from the leaves of collected from Chenzhou, Hunan Province, PR China. The main fatty acids (>5.0 %) of the strain were -C, C, C ω9, C, summed feature 5 (C ω6,9/C ante), C and C. MK-9(H), MK-9(H) and MK-9(H) were detected as respiratory quinones. The diagnostic cell-wall diamino acid was -diaminopimelic acid. Galactose, glucose and ribose were also present in the cell wall. The major polar lipids consisted of diphosphatidylglycerol, phosphatidyl ethanolamine, phosphatidylinositol mannosides and unidentified phospholipids. The DNA G+C content of the genome sequence, consisting of 8 860 963 bp, is 72.4 mol%. analysis based on 16S rRNA gene sequences revealed that the strain belongs to the genus , with 99.37, 99.03, 98.95, 98.68 and 98.67 % sequence similarity to ATCC 10762, DSM 44826, NBRC 13469, NRRL B-2218 and IFO 15206, respectively. Phylogenetic trees based on 16S rRNA gene and whole-genome sequences demonstrated that strain HUAS 3-15 formed a well‐supported cluster with ATCC 10762. Further genomic characterization through average nucleotide identity (ANIb/m) and digital DNA–DNA hybridization analysis between strain HUAS 3-15 and ATCC 10762 showed values of 90.62/92.55 % and 45.3 %, respectively, lower than the 95–96 % ANI threshold and 70.0 % cutoff used as guideline values for species delineation in bacteria. Furthermore, the differences between the strain and its phylogenomic neighbour in terms of physiological (e.g. sole carbon source growth) and chemotaxonomic (e.g. cellular fatty composition) characteristics further supported this conclusion. Consequently, we concluded that strain HUAS 3-15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HUAS 3-15 (=MCCC 1K08542=JCM 36274).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Cathaya argyrophylla , genomic characterization , Kitasatospora cathayae sp. nov. and phylogenetic analysis
Funding
This study was supported by the:
  • Innovation Team of Microbial Technology in Hunan University of Arts and Science (Award 202026)
    • Principle Award Recipient: YushuangLuo
  • Central guidance local science and technology development fund projects (Award 2023ZYC012)
    • Principle Award Recipient: YunWang
  • the Hunan Natural Science Foundation (Award 2023JJ50049)
    • Principle Award Recipient: WangYun
  • the Hunan Natural Science Foundation (Award 2023JJ50325)
    • Principle Award Recipient: LuoYushuang
  • the Hunan Natural Science Foundation (Award 2024JJ7300)
    • Principle Award Recipient: MoPing
  • the project of Hunan Provincial Education Department (Award 23B0659)
    • Principle Award Recipient: PingMo
  • the Innovation and Entrepreneurship Training Program for College Students
    • Principle Award Recipient: YaxiZheng
  • the Hunan Natural Science Foundation (Award 2023JJ30436)
    • Principle Award Recipient: PengXie
  • Hunan Provincial Natural Science Foundation (2023JJ40464)
    • Principle Award Recipient: ZhouZhibo
© 2024 The Authors
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Kitasatospora cathayae sp. nov., a novel endophytic actinomycete isolated from the leaves of Cathaya argyrophylla
Int J Syst Evol Microbiol 74, 006406 (2024); https://doi.org/10.1099/ijsem.0.006406
/content/journal/ijsem/10.1099/ijsem.0.006406
/content/journal/ijsem/10.1099/ijsem.0.006406
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