1887

Abstract

A rapidly growing nontuberculous mycobacterium was isolated from diseased koi carp in Niigata, Japan, which was identified as representing a novel species through whole genome sequence analysis. The bacterial isolates (NGTWS0302, NGTWS1803 and NGTWSNA01) were found to belong to the genus through phylogenetic analysis using whole genome sequences of mycobacteria species. The bacterial colony was smooth, moist and non-chromogenic on 1% Ogawa medium at 30 °C. In biochemical characteristic tests, the bacterial isolates showed positive reactions for catalase activity, Tween 80 hydrolysis and tellurite reduction. The isolates were sensitive to 2–4 µg ml ampicillin, kanamycin and rifampicin. Based on these results, we propose a novel species, sp. nov. The type strain is NGTWS1803 (=JCM 35117=ATCC TSD-289).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.006404
2024-05-29
2024-06-19
Loading full text...

Full text loading...

References

  1. Euzéby JP. List of Bacterial Names with Standing in Nomenclature: a folder available on the internet. Int J Syst Bacteriol 1997; 47:590–592 [View Article] [PubMed]
    [Google Scholar]
  2. Runyon EH. Anonymous mycobacteria in pulmonary disease. Med Clin North Am 1959; 43:273–290 [View Article] [PubMed]
    [Google Scholar]
  3. Gupta RS, Lo B, Son J. Phylogenomics and comparative genomic studies robustly support division of the genus Mycobacterium into an emended genus Mycobacterium and four novel genera. Front Microbiol 2018; 9:67 [View Article] [PubMed]
    [Google Scholar]
  4. Jacobs JM, Stine CB, Baya AM, Kent ML. A review of mycobacteriosis in marine fish. J Fish Dis 2009; 32:119–130 [View Article] [PubMed]
    [Google Scholar]
  5. Gauthier DT, Rhodes MW. Mycobacteriosis in fishes: a review. Vet J 2009; 180:33–47 [View Article] [PubMed]
    [Google Scholar]
  6. Aronson JD. Spontaneous tuberculosis in salt water fish. J Infect Dis 1926; 39:315–320 [View Article]
    [Google Scholar]
  7. Levi MH, Bartell J, Gandolfo L, Smole SC, Costa SF et al. Characterization of Mycobacterium montefiorense sp. nov., a novel pathogenic Mycobacterium from moray eels that is related to Mycobacterium triplex. J Clin Microbiol 2003; 41:2147–2152 [View Article] [PubMed]
    [Google Scholar]
  8. Rhodes MW, Kator H, Kotob S, van Berkum P, Kaattari I et al. Mycobacterium shottsii sp. nov., a slowly growing species isolated from Chesapeake Bay striped bass (Morone saxatilis). Int J Syst Evol Microbiol 2003; 53:421–424 [View Article] [PubMed]
    [Google Scholar]
  9. Rhodes MW, Kator H, McNabb A, Deshayes C, Reyrat J-M et al. Mycobacterium pseudoshottsii sp. nov., a slowly growing chromogenic species isolated from Chesapeake Bay striped bass (Morone saxatilis). Int J Syst Evol Microbiol 2005; 55:1139–1147 [View Article] [PubMed]
    [Google Scholar]
  10. Whipps CM, Butler WR, Pourahmad F, Watral VG, Kent ML. Molecular systematics support the revival of Mycobacterium salmoniphilum (ex Ross 1960) sp. nov., nom. rev., a species closely related to Mycobacterium chelonae. Int J Syst Evol Microbiol 2007; 57:2525–2531 [View Article] [PubMed]
    [Google Scholar]
  11. Pourahmad F, Cervellione F, Thompson KD, Taggart JB, Adams A et al. Mycobacterium stomatepiae sp. nov., a slowly growing, non-chromogenic species isolated from fish. Int J Syst Evol Microbiol 2008; 58:2821–2827 [View Article] [PubMed]
    [Google Scholar]
  12. Fukano H, Wada S, Kurata O, Katayama K, Fujiwara N et al. Mycobacterium stephanolepidis sp. nov., a rapidly growing species related to Mycobacterium chelonae, isolated from marine teleost fish, Stephanolepis cirrhifer. Int J Syst Evol Microbiol 2017; 67:2811–2817 [View Article] [PubMed]
    [Google Scholar]
  13. Machida Y, Tang BCC, Yamada M, Sato S, Nakajima K et al. Mycobacteriosis in cultured koi carp Cyprinus carpio caused by Mycobacterium paragordonae and two Mycolicibacterium spp. Aquaculture 2021; 539:736656 [View Article]
    [Google Scholar]
  14. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T et al. The RAST server: rapid annotations using subsystems technology. BMC Genomics 2008; 9:75 [View Article] [PubMed]
    [Google Scholar]
  15. Alikhan N-F, Petty NK, Ben Zakour NL, Beatson SA. BLAST Ring Image Generator (BRIG): simple prokaryote genome comparisons. BMC Genomics 2011; 12:402 [View Article] [PubMed]
    [Google Scholar]
  16. Pagnout C, Férard J-F, Poupin P. Characterization of IS1110-like sequences found in Mycobacterium species other than Mycobacterium avium. Res Microbiol 2006; 157:650–658 [View Article] [PubMed]
    [Google Scholar]
  17. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article] [PubMed]
    [Google Scholar]
  18. Meier-Kolthoff JP, Carbasse JS, Peinado-Olarte RL, Göker M. TYGS and LPSN: a database tandem for fast and reliable genome-based classification and nomenclature of prokaryotes. Nucleic Acids Res 2022; 50:D801–D807 [View Article] [PubMed]
    [Google Scholar]
  19. Rodriguez-R LM, Konstantinidis KT. Estimating coverage in metagenomic data sets and why it matters. ISME J 2014; 8:2349–2351 [View Article] [PubMed]
    [Google Scholar]
  20. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37:463–464 [View Article]
    [Google Scholar]
  21. Choo SW, Dutta A, Wong GJ, Wee WY, Ang MY et al. Comparative genomic analysis reveals a possible novel non-tuberculous Mycobacterium species with high pathogenic potential. PLoS One 2016; 11:e0150413 [View Article] [PubMed]
    [Google Scholar]
  22. Wattam AR, Davis JJ, Assaf R, Boisvert S, Brettin T et al. Improvements to PATRIC, the all-bacterial bioinformatics database and analysis resource center. Nucleic Acids Res 2017; 45:D535–D542 [View Article] [PubMed]
    [Google Scholar]
  23. Tan JL, Khang TF, Ngeow YF, Choo SW. A phylogenomic approach to bacterial subspecies classification: proof of concept in Mycobacterium abscessus. BMC Genomics 2013; 14:879 [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.006404
Loading
/content/journal/ijsem/10.1099/ijsem.0.006404
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error