1887

Abstract

Two Gram-negative bacteria, designated as strains LF1 and HM2-2, were isolated from an artificial pond in a honey farm at Hoengseong-gun, Gangwon-do, Republic of Korea. The 16S rRNA sequence analysis results revealed that strain LF1 belonged to the genus and had the highest sequence similarity to GH41-7 (99.0 %), CJ29 (98.9 %), and SYSU H10001 (98.2 %). Its growth occurred at 20–37 °C, at pH 5.0–12.0, and in the presence of 0–2% NaCl. The major fatty acids were iso-C, iso-C, and summed feature 9 (iso-C 9 and/or C 10-methyl). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The DNA G+C content was 67.5 mol%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain LF1 and species of the genus were 79.1–84.4% and 22.0–27.5 %, respectively. The 16S rRNA sequence analysis results revealed that strain HM2-2 belonged to the genus and was most closely related to II-D5 (98.9 %), JUR4 (98.4%), and II-B4 (98.4 %). Its growth occurred at 10–35 °C, at pH 5.0–11.0, and in the presence of 0–2% NaCl. The major fatty acids were C and summed feature 3 (C 7/C 6). The major polar lipid was phosphatidylethanolamine. The DNA G+C content was 59.9 mol%. The ANI and dDDH values between strain HM2-2 and its closely related strains were 75.1–83.0% and 20.4–26.4 %, respectively. Phenotypic, genomic, and phylogenetic data revealed that strains LF1 and HM2-2 represent novel species in the genera and , for which the names sp. nov. and sp. nov. are proposed, respectively. The type strain of is LF1 (=KACC 23251=TBRC 17648), and that of is HM2-2 (=KACC 23250=TBRC 17649).

Funding
This study was supported by the:
  • National Research Foundation of Korea (Award 2022R1F1A1070108)
    • Principle Award Recipient: TaegunSeo
  • National Institute of Biological Resources (Award NIBR202304204)
    • Principle Award Recipient: TaegunSeo
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2024-05-28
2024-06-19
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