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Abstract

A Gram-stain-negative bacterium, designated LG-2, was isolated from sludge collected at a pesticide-manufacturing factory in Jiangsu Province, PR China. Cells of strain LG-2 were strictly aerobic, non-motile and spherical. Growth was observed at 15–42 °C (optimum, 30 °C), pH 6.0–9.0 (optimum, pH 7.0) and 0–3.0 % (w/v) NaCl (optimum, 1.0 %). LG–2 showed 95.5–96.9 % 16S rRNA sequence similarity to type strains in the genera , , , and of the family . The phylogenomic tree indicated that strain LG-2 was clustered in the family and formed a clade with IMT-305, while the phylogenetic trees based on 16S rRNA gene sequences indicated that strain LG-2 formed a distinct clade within the family . The average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values between LG-2 and its closely related type strains in the genera , , , and were 70.8–75.3, 18.9–23.7 and 59.6 %–69.3 %, respectively. The major cellular fatty acids were C, C cyclo, summed feature 3 (C ω7 and/or C ω6), summed feature 8 (C ω7 and/or C ω6) and summed feature 2 (C aldehyde and/or unknown 10.928). The predominant menaquinone was Q-8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, two aminophospholipids, three aminolipids and nine unknown polar lipids. The genome size of strain LG-2 was 3.2 Mb and the DNA G+C content was 63.4 mol%. On the basis of the phenotypic, phylogenetic and genomic results from this study, strain LG-2 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed, with strain LG-2 (=KCTC 8084= CCTCC AB 2023123) as the type strain.

Funding
This study was supported by the:
  • Water Resources Department of Guangdong Province (Award BM2022019)
    • Principle Award Recipient: LuyaoRuan
  • Natural Science Foundation Project of Chongqing, Chongqing Science and Technology Commission (Award 32170128)
    • Principle Award Recipient: NotApplicable
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2024-05-20
2024-06-19
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