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Abstract

A Gram-stain-positive actinomycete, designated REN17, was isolated from fermented grains of Baijiu collected from Sichuan, PR China. It exhibited branched substrate mycelia and a sparse aerial mycelium. The optimal growth conditions for REN17 were determined to be 28 °C and pH 7, with a NaCl concentration of 0 % (w/v). -Diaminopimelic acid was the diagnostic amino acid of the cell-wall peptidoglycan and the polar lipids were composed of phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid, two unidentified lipids and four unidentified glycolipids. The predominant menaquinone was MK-9 (H), MK-9 (H), MK-9 (H) and MK-9 (H). The major fatty acids were iso-C. The 16S rRNA sequence of REN17 was most closely related to those of SUN 51 (99.8 %), BH-SS-21 (99.6 %) and JCM 4521 (98.9 %). The digital DNA–DNA hybridization, average nucleotide identity and average amino acid identify values between REN17 and its closest replated strain, of SUN 51, were 35.9, 88.9 and 87.3 %, respectively. Therefore, REN17 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is REN17 (=GDMCC 4.193=JCM 34712). While exploring the function of the strain, REN17 was found to possess the ability to transform major ginsenosides of (Burk.) F.H. Chen (Araliaceae) into minor ginsenoside through HPLC separation, which was due to the presence of β-glucosidase. The recombinant β-glucosidase was constructed and purified, which could produce minor ginsenosides of Rg3 and C-K. Finally, the enzymatic properties were characterized.

Funding
This study was supported by the:
  • Major Science and Technology Project of Yunnan (Award 202102AE090042)
    • Principle Award Recipient: QingRen
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/content/journal/ijsem/10.1099/ijsem.0.006392
2024-05-20
2024-06-19
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