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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 5

gen. nov., sp. nov. and sp. nov., isolated from desert soil, and reclassification of as comb. nov. and as gen.nov., comb. nov. No Access

Abstract

Two novel bacterial strains, designated as SYSU D00823 and SYSU D00873, were isolated from sandy soil of the Gurbantunggut Desert in Xinjiang, north-west China. SYSU D00823 and SYSU D00873 shared 99.0 % 16S rRNA gene sequence identity, and were both most closely related to 12157 with 96.1 % and 96.0 % similarities, respectively. Phylogenetic and phylogenomic analyses revealed that the two isolates and 12157 formed a separate distinct cluster in a stable subclade with the nearby species 1-32, as well as the genera and . Furthermore, 1-32 formed a separate deep-branching lineage and did not form a cluster with members of the genus . The average nucleotide identity and digital DNA–DNA hybridization values between SYSU D00823 and SYSU D00873 and related species were well below the thresholds for species delineation (<81.0 % and <24.0 %, respectively). The genomes of SYSU D00823 and SYSU D00873 were 6.19 and 6.43 Mbp in size with 40.4 % and 40.5 % DNA G+C contents, respectively. The predominant fatty acids (>10 %) of SYSU D00823 and SYSU D00873 were iso-C, iso-C 3-OH and summed feature 3 (C 7 and/or C 6). Menaquinone-7 was the only respiratory quinone. The major polar lipids were phosphatidylethanolamine, glycosphingolipid, aminoglycolipid/glycolipid, aminophospholipid and three or four unidentified polar lipids. These data indicated that strains SYSU D00823 and SYSU D00873 should be assigned to two novel species of a new genus within the family , for which the names gen. nov., sp. nov. and sp. nov. are proposed. The type strains are SYSU D00823 (=CGMCC 1.18630=MCCC 1K04973=KCTC 82278) and SYSU D00873 (=CGMCC 1.18629=MCCC 1K04974=KCTC 82281), respectively. Accordingly, the reclassification of as comb. nov., and as gen. nov., comb. nov. are also proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): desert soil , Desertivirga arenae , Desertivirga brevis , Pedobacter , polyphasic taxonomy and whole-genome sequencing
Funding
This study was supported by the:
  • Xinjiang Uygur Autonomous Region regional coordinated innovation project (Award 2021E01018)
    • Principle Award Recipient: Wen-JunLi
  • National Natural Science Foundation of China (Award 32000005)
    • Principle Award Recipient: LeiDong
  • National Natural Science Foundation of China (Award 32061143043)
    • Principle Award Recipient: Wen-JunLi
  • Guangdong Basic and Applied Basic Research Foundation (Award 2023A1515012020)
    • Principle Award Recipient: ShuaiLi
  • Third Xinjiang Scientific Expedition Program (Award 2022xjkk1200)
    • Principle Award Recipient: Wen-JunLi
© 2024 Sun Yat-Sen University
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Desertivirga arenae gen. nov., sp. nov. and Desertivirga brevis sp. nov., isolated from desert soil, and reclassification of Pedobacter xinjiangensis as Desertivirga xinjiangensis comb. nov. and Pedobacter mongoliensis as Paradesertivirga mongoliensis gen.nov., comb. nov.
Int J Syst Evol Microbiol 74, 006390 (2024); https://doi.org/10.1099/ijsem.0.006390
/content/journal/ijsem/10.1099/ijsem.0.006390
/content/journal/ijsem/10.1099/ijsem.0.006390
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