1887

Abstract

Two novel bacterial strains, designated as SYSU D00823 and SYSU D00873, were isolated from sandy soil of the Gurbantunggut Desert in Xinjiang, north-west China. SYSU D00823 and SYSU D00873 shared 99.0 % 16S rRNA gene sequence identity, and were both most closely related to 12157 with 96.1 % and 96.0 % similarities, respectively. Phylogenetic and phylogenomic analyses revealed that the two isolates and 12157 formed a separate distinct cluster in a stable subclade with the nearby species 1-32, as well as the genera and . Furthermore, 1-32 formed a separate deep-branching lineage and did not form a cluster with members of the genus . The average nucleotide identity and digital DNA–DNA hybridization values between SYSU D00823 and SYSU D00873 and related species were well below the thresholds for species delineation (<81.0 % and <24.0 %, respectively). The genomes of SYSU D00823 and SYSU D00873 were 6.19 and 6.43 Mbp in size with 40.4 % and 40.5 % DNA G+C contents, respectively. The predominant fatty acids (>10 %) of SYSU D00823 and SYSU D00873 were iso-C, iso-C 3-OH and summed feature 3 (C 7 and/or C 6). Menaquinone-7 was the only respiratory quinone. The major polar lipids were phosphatidylethanolamine, glycosphingolipid, aminoglycolipid/glycolipid, aminophospholipid and three or four unidentified polar lipids. These data indicated that strains SYSU D00823 and SYSU D00873 should be assigned to two novel species of a new genus within the family , for which the names gen. nov., sp. nov. and sp. nov. are proposed. The type strains are SYSU D00823 (=CGMCC 1.18630=MCCC 1K04973=KCTC 82278) and SYSU D00873 (=CGMCC 1.18629=MCCC 1K04974=KCTC 82281), respectively. Accordingly, the reclassification of as comb. nov., and as gen. nov., comb. nov. are also proposed.

Funding
This study was supported by the:
  • Xinjiang Uygur Autonomous Region regional coordinated innovation project (Award 2021E01018)
    • Principle Award Recipient: Wen-JunLi
  • National Natural Science Foundation of China (Award 32000005)
    • Principle Award Recipient: LeiDong
  • National Natural Science Foundation of China (Award 32061143043)
    • Principle Award Recipient: Wen-JunLi
  • Guangdong Basic and Applied Basic Research Foundation (Award 2023A1515012020)
    • Principle Award Recipient: ShuaiLi
  • Third Xinjiang Scientific Expedition Program (Award 2022xjkk1200)
    • Principle Award Recipient: Wen-JunLi
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2024-05-16
2024-06-19
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