1887

Abstract

A bacterial strain designated MMS21-TAE1-1, capable of degrading paraoxon, was isolated from red pepper soil (36° 25′ 26.0″ N, 126° 25′ 47.0″ E) and subjected to polyphasic taxonomic characterisation. MMS21-TAE1-1 was an aerobic, non-motile and Gram-stain-positive bacterium. MMS21-TAE1-1 showed growth at 10–37 °C (optimum, 30 °C), at pH 4–10 (optimum, pH 7) and in the presence of 0–6 % NaCl (optimum, 0 %). On the basis of the results of 16S rRNA gene sequence analysis, MMS21-TAE1-1 could be assigned to the genus and shared the highest sequence similarities with NBRC 12136 (99.72 %), then with G2-1 (99.65 %) and DSM 20138 (99.17 %). However, the results of genome-based comparison using orthologous average nucleotide identity (orthoANI) and digital DNA–DNA hybridisation indicated that MMS21-TAE1-1 could be readily distinguished from all species of the genus with validly published names. The predominant menaquinone of MMS21-TAE1-1 was MK-9(H). The diagnostic polar lipids were diphosphatidylglycerol and phosphatidylinositol, and unidentified glycolipids were also present. The major fatty acids were anteiso-C, anteiso-C, iso-C and iso-C. The chemotaxonomic properties of MMS21-TAE1-1 were generally consistent with those of members of the genus . The genome of MMS21-TAE1-1 contained genes related to degradation of aromatic compounds. It is evident from the results of this study that strain MMS21-TAE1-1 merits recognition as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MMS21-TAE1-1 (=KCTC 49652 = LMG 32368).

Funding
This study was supported by the:
  • National Institute of Biological Resources (NIBR)
    • Principle Award Recipient: SeungBum Kim
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.006389
2024-05-16
2024-06-18
Loading full text...

Full text loading...

References

  1. Busse H-J. Review of the taxonomy of the genus Arthrobacter, emendation of the genus Arthrobacter sensu lato, proposal to reclassify selected species of the genus Arthrobacter in the novel genera Glutamicibacter gen. nov., Paeniglutamicibacter gen. nov., Pseudoglutamicibacter gen. nov., Paenarthrobacter gen. nov. and Pseudarthrobacter gen. nov., and emended description of Arthrobacter roseus. Int J Syst Evol Microbiol 2016; 66:9–37 [View Article]
    [Google Scholar]
  2. Kang Z, Yang Y, Wang C, Kang Y, Wang T et al. Atrazine decontamination by a newly screened psychrotroph Paenarthrobacter sp. KN0901 in an aquatic system: metabolic pathway, kinetics, and hydroponics experiment. J Hazard Mater 2023; 457:131764 [View Article] [PubMed]
    [Google Scholar]
  3. Zhang M, Ren Y, Jiang W, Wu C, Zhou Y et al. Comparative genomic analysis of iprodione-degrading Paenarthrobacter strains reveals the iprodione catabolic molecular mechanism in Paenarthrobacter sp. strain YJN-5. Environ Microbiol 2021; 23:1079–1095 [View Article] [PubMed]
    [Google Scholar]
  4. Zhang M, Jiang W, Gao S, Zhu Q, Ke Z et al. Degradation of dimethachlon by a newly isolated bacterium Paenarthrobacter sp. strain JH-1 relieves its toxicity against Chlorella ellipsoidea. Environ Res 2022; 208:112706 [View Article] [PubMed]
    [Google Scholar]
  5. Deshpande LS, Phillips K, Huang B, DeLorenzo RJ. Chronic behavioral and cognitive deficits in a rat survival model of paraoxon toxicity. Neurotoxicology 2014; 44:352–357 [View Article] [PubMed]
    [Google Scholar]
  6. Kim YS, Roh SG, Kim SB. Gordonia insulae sp. nov., isolated from an island soil. Int J Syst Evol Microbiol 2020; 70:2079–2083 [View Article] [PubMed]
    [Google Scholar]
  7. Jeon Y-S, Lee K, Park S-C, Kim B-S, Cho Y-J et al. EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. Int J Syst Evol Microbiol 2014; 64:689–691 [View Article] [PubMed]
    [Google Scholar]
  8. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article] [PubMed]
    [Google Scholar]
  9. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article] [PubMed]
    [Google Scholar]
  10. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article] [PubMed]
    [Google Scholar]
  11. Kim M, Oh H-S, Park S-C, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [View Article] [PubMed]
    [Google Scholar]
  12. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012; 19:455–477 [View Article] [PubMed]
    [Google Scholar]
  13. Tatusova T, DiCuccio M, Badretdin A, Chetvernin V, Nawrocki EP et al. NCBI prokaryotic genome annotation pipeline. Nucleic Acids Res 2016; 44:6614–6624 [View Article] [PubMed]
    [Google Scholar]
  14. Lee I, Ouk Kim Y, Park SC, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016; 66:1100–1103 [View Article] [PubMed]
    [Google Scholar]
  15. Meier-Kolthoff JP, Klenk HP, Göker M. Taxonomic use of DNA G+C content and DNA–DNA hybridization in the genomic age. Int J Syst Evol Microbiol 2014; 64:352–356 [View Article] [PubMed]
    [Google Scholar]
  16. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinform 2013; 14:60 [View Article] [PubMed]
    [Google Scholar]
  17. Xu P, Li W-J, Tang S-K, Zhang Y-Q, Chen G-Z et al. Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family “Oxalobacteraceae” isolated from China. Int J Syst Evol Microbiol 2005; 55:1149–1153 [View Article] [PubMed]
    [Google Scholar]
  18. Cowan ST, Steel KJ. Manual for the Identification of Medical Bacteria Cambridge University Press; 1965
    [Google Scholar]
  19. Vandamme P, Vancanneyt M, Pot B, Mels L, Hoste B et al. Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol 1992; 42:344–356 [View Article] [PubMed]
    [Google Scholar]
  20. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. In MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  21. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  22. Collins M. Isoprenoid quinone analyses in bacterial classification and identification. In Goodfellow M, Minnikin D. eds Chemical Methods in Bacterial Systematics London: Academic Press; 1985 pp 267–287
    [Google Scholar]
  23. Oyuntsetseg B, Lee HB, Kim SB. Amycolatopsis mongoliensis sp. nov., a novel actinobacterium with antifungal activity isolated from a coal mining site in Mongolia. Int J 2024; 74: [View Article]
    [Google Scholar]
  24. Collins MD, Jones D, Kroppenstedt RM. Reclassification of Corynebacterium ilicis (Mandel, Guba and Litsky) in the genus Arthrobacter, as Arthrobacter ilicis comb. nov. Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I Abt Originale C: Allgemeine, angewandte und ökologische Mikrobiologie 1981; 2:318–323 [View Article]
    [Google Scholar]
  25. Kotoučková L, Schumann P, Durnová E, Spröer C, Sedláček I et al. Arthrobacter nitroguajacolicus sp. nov., a novel 4-nitroguaiacol-degrading actinobacterium. Int J Syst Evol Microbiol 2004; 54:773–777 [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.006389
Loading
/content/journal/ijsem/10.1099/ijsem.0.006389
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error