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Abstract

Two rod-shaped, obligate anaerobic, Gram-stain-positive bacteria isolated from the pig faeces were designated YH-ols2216 and YH-ols2217. Analysis of 16S rRNA gene sequences revealed that these isolates were most related to the members of the family , within the order , and KCTC 25474 with 92.0 and 92.5% similarities, respectively. The 16S rRNA gene sequence similarity within isolates was 99.9 %; and those between isolates YH-ols2216 and YH-ols2217, and DSM 20586, the type species of the type genus within the family , were 88.5 and 88.7 %, respectively. Those between isolates and PW2, the type species of the type genus within the family , were 88.7 and 89.1 %, respectively. The multi-locus sequence tree revealed that the isolates, alongside the genera and , formed a distinct cluster between the families and . The average nucleotide identities and digital DNA–DNA hybridization values for the isolates and their most closely related strains ranged from 67.7 to 76.2 % and from 18.4 to 23.3 %, respectively. The main cellular fatty acids of the isolates were C DMA, C 9, C 12OH, C, and C. The cell wall contained the peptidoglycan -diaminopimelic acid. Lactate was the main end-product of the isolates. The major polar lipids of isolate YH-ols2217 were aminophospholipid, aminolipids, and lipids. Menaquinones were not identified in the cells of the isolates. The DNA G+C contents of isolates YH-ols2216 and YH-ols2217 were 67.5 and 67.6 mol%, respectively. Considering these chemotaxonomic, phenotypic, and phylogenetic properties, fam. nov. is proposed within the order . YH-ols2216 (=KCTC 25708=NBRC 116429) and YH-ols2217 (=KCTC 25709=NBRC 116430) represent a novel taxon within this new family and the name gen. nov., sp. nov. is proposed. In addition, the genera and are transferred to the family fam. nov.

Funding
This study was supported by the:
  • the National Research Foundation of Korea (Award 2021R1A2C2009051)
    • Principle Award Recipient: Joong-KiKook
  • Korea Research Institute of Bioscience and Biotechnology (Award KGS1022423)
    • Principle Award Recipient: Young-HyoChang
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2024-05-10
2025-02-15
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