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Abstract

A Gram-stain-negative and rod-shaped bacterium, designated strain CY04, was isolated from a sediment sample collected from the Yellow Sea. CY04 exhibited the highest 16S rRNA gene sequence similarity of 98.7 % to CY05, followed by the similarities of 98.6 %, 98.0 and 98.0 % to DSW4-44, W43 and QS115 respectively. Phylogenetic analysis based on 16S rRNA gene and phylogenomic analysis based on genome sequences revealed that CY04 formed a robust cluster with CY05, DSW4-44, W43 and QS115. Calculated digital DNA–DNA hybridisation and average nucleotide identity values between CY04 and its closely related species were 22.2–23.7 % and 79.0–81.2 % respectively. Cells of CY04 were strictly aerobic, non-motile and positive for catalase, oxidase and denitrification. CY04 harboured a set of genes encoding the enzymes involved in denitrification. Growth occurred at 10–30 °C (optimum, 20 °C), at pH 6.5–9.5 (optimum, pH 8.0) and with 1–6 % (w/v) (optimum, 2.5 %,) NaCl. The major component of the fatty acids was summed feature 8 (Cω7 and/or Cω6). The isoprenoid quinone was Q-10. Results of the phenotypic, chemotaxonomic and molecular study indicate that strain CY04 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CY04 (=MCCC 1K08635=KCTC 62199). It is also proposed that and should be reclassified as comb. nov. and nom. nov. An emended description of the genus is also proposed.

Funding
This study was supported by the:
  • Projects of Medical and Health Technology Development Program in Shandong Province (Award 202202040316)
    • Principle Award Recipient: CaixingShi
  • Lin He's New Medicine and Clinical Translation Academician Workstation Research Fund (Award JYHL2022MS12)
    • Principle Award Recipient: AngLiu
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2024-05-10
2024-05-26
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