1887

Abstract

A Gram-negative, rod-shaped, non-motile and strictly aerobic strain, designated NBU2979, was isolated from a coastal mudflat located on Meishan Island in the East China Sea. Strain NBU2979 grew optimally at 32 °C, with 2.0 % NaCl (w/v) and at pH 7.0–7.5. The predominant fatty acid (>10 %) was iso-C. The major polar lipids included phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidyldimethylethanolamine, phosphatidylcholine, an unidentified glycolipid, two unidentified aminophospholipids, an unidentified phospholipid and an unidentified lipid. The only respiratory quinone was ubiquinone-8. Comparative analysis of 16S rRNA gene sequences showed that strain NBU2979 exhibited highest similarity to F2 (98.0 %), S1101 (97.5 %), KMM 3900 (96.6 %), 3539 (95.5 %), sw153 (95.2 %) and S6413 (94.9 %). Phylogenetic analyses indicated that strain NBU2979 clustered with the genus and was closely related to strain F2. The average nucleotide identity and digital DNA–DNA hybridization values between strain NBU2979 and related species of genus were well below the threshold limit for prokaryotic species delineation. The DNA G+C content of strain NBU2979 was 51.6 mol%. Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU2979 (=KCTC 82911=MCCC 1K06402) is considered to be a representative of a novel species in the genus , for which the name sp. nov. is proposed.

Funding
This study was supported by the:
  • Higher Education Discipline Innovation Project (Award D16013)
    • Principle Award Recipient: weiyanzhang
  • Scientific Research Foundation for Returned Scholars of Ministry of Education (Award IF2021087)
    • Principle Award Recipient: weiyanzhang
  • Science and Technology Development Center, Ministry of Education (Award 2021Z046)
    • Principle Award Recipient: weiyanzhang
  • Natural Science Foundation of Zhejiang Province (Award LGF22C010001)
    • Principle Award Recipient: weiyanzhang
  • National Natural Science Foundation of China (Award 32100001, 42176101)
    • Principle Award Recipient: weiyanzhang
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2024-05-24
2024-06-19
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