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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 5

sp. nov., proposed for clade V organisms of No Access

Abstract

A set of 25 strains belonging to clade V of mainly isolated from cattle was investigated and is proposed to represent sp. nov. The species can be separated from the other validly published species of the genus by pheno- and genotype. Only indole separates and while two to seven characters separate from other species of . Thirteen strains belonging to biogroups 6, 7, 8C, 9, 10, 12 and UG5 formed a monophyletic group based on 16S rRNA gene sequence comparisons with 98–100 % similarity. Eight of these strains were further included in the whole genome comparison. Digital DNA–DNA hybridization showed that the similarities between the suggested type strain M14.4 and the other strains of were 62.9 % or higher. The average nucleotide identity was 95.5 % or higher between M14.4 and the other strains of the species. The gene sequence similarity was 95–100 % within . MALDI-TOF allowed a clear separation from other species further supporting classification as a novel species and making it the diagnostic identification tool of choice for . The type strain is M14.4 (=CCUG 77347=DSM 116804) isolated from a cattle tongue in Scotland.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): biogroup , cAMP , MALDI-TOF , MEE and ruminant
Funding
This study was supported by the:
  • Danish Agricultural and Veterinary Research Council
    • Principle Award Recipient: NotApplicable
© 2024 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.006370
2024-05-13
2025-05-22
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Mannheimia indoligenes sp. nov., proposed for clade V organisms of Mannheimia
Int J Syst Evol Microbiol 74, 006370 (2024); https://doi.org/10.1099/ijsem.0.006370
/content/journal/ijsem/10.1099/ijsem.0.006370
/content/journal/ijsem/10.1099/ijsem.0.006370
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