Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 5

f.a. sp. nov., a novel ascomycetous yeast species isolated from flowers of in India No Access

Abstract

Two isolates representing a novel species of the genus were obtained in India from nectar of flowers of , an ornamental exotic species native to Central and South America. Phylogenetic analyses of the D1/D2 domain of the 26S large subunit (LSU) rRNA gene, internal transcribed spacer (ITS) region, and physiological characteristics, supported the recognition of the novel species, that we designate sp. nov (MycoBank no. MB851709), with MCC 9929 as the holotype and PYCC 10003 as the isotype. Considering pairwise sequence similarity, the type strain of the novel species differs from the type strain of the most closely related species, CBS 8459, by 16 nucleotide substitutions and two gaps (3.9 % sequence variation) in the D1/D2 region (560 bp compared) and 28 nucleotide substitutions and five gaps (7.22 % sequence variation) in the ITS region (444 bp compared).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ascomycetous yeast , flower nectar microbiology , India , new yeast species and Wickerhamiella lachancei
Funding
This study was supported by the:
  • Fundação para a Ciência e a Tecnologia (Award UIDB/04378/2020 (UCIBIO), LA/P/0140/2020 (i4HB))
    • Principle Award Recipient: JoséPaulo Sampaio
  • Department of Biotechnology, Ministry of Science and Technology, India (Award BT/COORD.II/01/03/2016 (NCMR))
    • Principle Award Recipient: AvinashSharma
© 2024 The Authors
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.006365
2024-05-07
2025-04-18
Loading full text...

Full text loading...

References

  1. de Vega C, Albaladejo RG, Guzmán B, Steenhuisen S-L, Johnson SD et al. Flowers as a reservoir of yeast diversity: description of Wickerhamiella nectarea f.a. sp. nov., and Wickerhamiella natalensis f.a. sp. nov. from South African flowers and pollinators, and transfer of related Candida species to the genus Wickerhamiella as new combinations. FEMS Yeast Res 2017; 17:fox 054 [View Article] [PubMed]
     [Google Scholar]
  2. Lachance MA, Boekhout T, Scorzetti G. Candida Berkhout. In The Yeasts, A Taxonomic Study, 5th edn. Amsterdam: Elsevier; 2011 pp 987–1278
     [Google Scholar]
  3. Amoikon TL, Grondin C, Djéni TN, Jacques N. Starmerella reginensis f.a., sp. nov. and Starmerella kourouensis f.a., sp. nov., isolated from flowers in French Guiana. Int J Syst Evol Microbiol 2018; 68:2299–2305 [View Article]
     [Google Scholar]
  4. Lachance MA, Vale HMM, Sperandio EM, Carvalho AOS, Santos ARO et al. Wickerhamiella dianesei f.a., sp. nov. and Wickerhamiella kurtzmanii f.a., sp. nov., two yeast species isolated from plants and insects. Int J Syst Evol Microbiol 2018; 68:3351–3355 [View Article]
     [Google Scholar]
  5. Santos ARO, Leon MP, Barros KO, Freitas LFD, Hughes AFS et al. Starmerella camargoi f.a., sp. nov., Starmerella ilheusensis f.a., sp. nov., Starmerella litoralis f.a., sp. nov., Starmerella opuntiae f.a., sp. nov., Starmerella roubikii f.a., sp. nov. and Starmerella vitae f.a., sp. nov., isolated from flowers and bees, and transfer of related Candida species to the genus Starmerella as new combinations. Int J Syst Evol Microbiol 2018; 68:1333–1343 [View Article] [PubMed]
     [Google Scholar]
  6. Kanpiengjai A, Kodchasee P, Unban K, Kumla J, Lumyong S et al. Three new yeast species from flowers of Camellia sinensis var. assamica collected in Northern Thailand and their tannin tolerance characterization. Front Microbiol 2023; 16:1043430 [View Article] [PubMed]
     [Google Scholar]
  7. Seike T, Takekata H, Uchida Y, Sakata N, Furusawa C et al. Wickerhamiella bidentis sp. nov., a novel yeast species isolated from flowers and insects in Japan. Int J Syst Evol Microbiol 2023; 8:005739 [View Article] [PubMed]
     [Google Scholar]
  8. Lachance MA, Kurtzman CP. Wickerhamiella van der Walt (1973). In The Yeasts, A Taxonomic Study, 5th edn. Amsterdam: Elsevier; 1973 p 2011
     [Google Scholar]
  9. Belloch C, Pelaez AI, Sánchez J, Kurtzman CP. Wickerhamiella verensis f.a. sp. nov., a novel yeast species isolated from subsoil groundwater contaminated with hydrocarbons and from a human infection. Int J Syst Evol Microbiol 2020; 70:2420–2425 [View Article] [PubMed]
     [Google Scholar]
  10. Avchar R, Groenewald M, Baghela A. Wickerhamiella shivajii sp. nov., a thermotolerant yeast isolated from distillery effluent. Int J Syst Evol Microbiol 2019; 69:3262–3267 [View Article] [PubMed]
     [Google Scholar]
  11. Nicolson SW, Thornburg RW. Nectar chemistry. In Nectaries and Nectar vol 18 Dordrecht: Springer; 2007 pp 215–264 [View Article]
     [Google Scholar]
  12. Roy R, Schmitt AJ, Thomas JB, Carter CJ. Nectar biology: from molecules to ecosystems. Plant Sci 2017; 262:148–164 [View Article] [PubMed]
     [Google Scholar]
  13. Herrera CM, Pérez R, Alonso C. Extreme intraplant variation in nectar sugar composition in an insect-pollinated perennial herb. Am J Bot 2006; 93:575–581 [View Article] [PubMed]
     [Google Scholar]
  14. Vannette RL, Fukami T. Nectar microbes can reduce secondary metabolites in nectar and alter effects on nectar consumption by pollinators. Ecology 2016; 97:1410–1419 [View Article] [PubMed]
     [Google Scholar]
  15. Tiwari S, Jadhav R, Avchar R, Lanjekar V, Datar M et al. Nectar yeast community of tropical flowering plants and assessment of their osmotolerance and xylitol-producing potential. Curr Microbiol 2022; 79:1–2 [View Article]
     [Google Scholar]
  16. Suh SO, Blackwell M, Kurtzman CP, Lachance MA. Phylogenetics of Saccharomycetales, the ascomycete yeasts. Mycologia 2006; 98:1006–1017 [View Article] [PubMed]
     [Google Scholar]
  17. Brysch-Herzberg M. Ecology of yeasts in plant-bumblebee mutualism in Central Europe. FEMS Microbiol Ecol 2004; 50:87–100 [View Article] [PubMed]
     [Google Scholar]
  18. Herrera CM, De Vega C, Canto A, Pozo MI. Yeasts in floral nectar: a quantitative survey. Ann Bot 2009; 103:1415–1423 [View Article] [PubMed]
     [Google Scholar]
  19. Barnett JA, Payne RW, Yarrow D. Yeast, Characteristics and Identification Cambridge University Press; 2000 p 1150
     [Google Scholar]
  20. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In The Yeasts, A Taxonomic Study, 5th edn. Amsterdam: Elsevier; 2011 pp 87–110
     [Google Scholar]
  21. Aamir S, Sutar S, Singh SK, Baghela A. A rapid and efficient method of fungal genomic DNA extraction, suitable for PCR based molecular methods. PPQ 2015; 5:74–81 [View Article]
     [Google Scholar]
  22. Ramírez-Castrillón M, Mendes SDC, Inostroza-Ponta M, Valente P. (GTG)5 MSP-PCR fingerprinting as a technique for discrimination of wine associated yeasts?. PLoS One 2014; 9:e105870 [View Article] [PubMed]
     [Google Scholar]
  23. White TJ, Bruns S, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: Guide Meth Appl 1990; 18:315–322
     [Google Scholar]
  24. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998; 73:331–371 [View Article] [PubMed]
     [Google Scholar]
  25. Kurtzman CP, Robnett CJ. Phylogenetic relationships among yeasts of the “Saccharomyces complex” determined from multigene sequence analyses. FEMS Yeast Res 2003; 3:417–432 [View Article] [PubMed]
     [Google Scholar]
  26. Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004; 32:1792–1797 [View Article] [PubMed]
     [Google Scholar]
  27. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article] [PubMed]
     [Google Scholar]
  28. Tamura K. Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G+C-content biases. Mol Biol Evol 1992; 9:678–687 [View Article] [PubMed]
     [Google Scholar]
  29. Lachance MA, Starmer WT. Ecology and yeasts. In The Yeasts, A Taxonomic Study, 4th edn. Amsterdam: Elsevier; 1998 pp 21–30
     [Google Scholar]
  30. Rosa CA, Morais PB, Lachance MA, Santos RO, Melo WG et al. Wickerhamomyces queroliae sp. nov. and Candida jalapaonensis sp. nov., two yeast species isolated from Cerrado ecosystem in North Brazil. Int J Syst Evol Microbiol 2009; 59:1232–1236 [View Article]
     [Google Scholar]
  31. Badotti F, Silva PAB, Mendonça MC, Gomes FCO, Morais PB et al. Wickerhamiella dulcicola sp. nov. and Wickerhamiella cachassae sp. nov., yeasts isolated from cachaça fermentation in Brazil. Int J Syst Evol Microbiol 2013; 63:1169–1173 [View Article] [PubMed]
     [Google Scholar]
  32. Liu XJ, Wang Y, Ren YC, Hui FL. Wickerhamiella brachini f.a., sp. nov., Wickerhamiella pterostichi f.a., sp. nov. and Wickerhamiella qilinensis f.a., sp. nov., three yeast species isolated from insects. Int J Syst Evol Microbiol 2016; 66:3995–4001 [View Article] [PubMed]
     [Google Scholar]
  33. Khunnamwong P, Kingphadung K, Lomthong T, Kanpiengjai A, Khanongnuch C et al. Wickerhamiella nakhonpathomensis f.a. sp. nov., a novel ascomycetous yeast species isolated from a mushroom and a flower in Thailand. Int J Syst Evol Microbiol 2022; 72:1 [View Article] [PubMed]
     [Google Scholar]
  34. Lachance MA, Rosa CA, Starmer WT, Schlag-Edler BI, Barker JS et al. Wickerhamiella australiensis, Wickerhamiella cacticola, Wickerhamiella occidentalis, Candida drosophilae and Candida lipophila, five new related yeast species from flowers and associated insects. Int J Syst Evol Microbiol 1998; 48:1431–1443 [View Article] [PubMed]
     [Google Scholar]
  35. Barbosa AC, Morais CG, Morais PB, Rosa LH, Pimenta RS et al. Wickerhamiella pagnoccae sp. nov. and Candida tocantinsensis sp. nov., two ascomycetous yeasts from flower bracts of Heliconia psittacorum (Heliconiaceae). Int J Syst Evol Microbiol 2012; 62:459–464 [View Article] [PubMed]
     [Google Scholar]
  36. Dayo-Owoyemi I, Rosa CA, Rodrigues A, Pagnocca FC. Wickerhamiella kiyanii f.a., sp. nov. and Wickerhamiella fructicola f.a., sp. nov., two yeasts isolated from native plants of Atlantic rainforest in Brazil. Int J Syst Evol Microbiol 2014; 64:2152–2158 [View Article] [PubMed]
     [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.006365
Loading
Wickerhamiella lachancei f.a. sp. nov., a novel ascomycetous yeast species isolated from flowers of Lantana camara in India
Int J Syst Evol Microbiol 74, 006365 (2024); https://doi.org/10.1099/ijsem.0.006365
/content/journal/ijsem/10.1099/ijsem.0.006365
/content/journal/ijsem/10.1099/ijsem.0.006365
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error