1887

Abstract

The use of algae as feedstock for industrial purposes, such as in bioethanol production, is desirable. During a search for new agarolytic marine bacteria, a novel Gram-stain-negative, strictly aerobic, and agarolytic bacterium, designated as TS8, was isolated from algae in the harbour of the island of Susak, Croatia. The cells were rod-shaped and motile. The G+C content of the sequenced genome was 38.6 mol%. Growth was observed at 11–37 °C, with 0.5–13 % (w/v) NaCl, and at pH 6.0–9.0. The main fatty acids were summed feature 3 (C 6 and/or C 7), summed feature 8 (C 7 and/or C 6), and C. The main respiratory quinone was ubiquinone-8. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. Analysis of 16S rRNA gene sequences indicated that the newly isolated strain belongs to the genus . Based on 16S rRNA gene sequence data, strain TS8 is closely related to D2 (95.7 %), YM01 (95.0 %), and Q1 (93.2 %). Digital DNA–DNA hybridization values between TS8 and the other strains were below 25 %. Based on genotypic, phenotypic, and phylogenetic data, strain TS8 represents a new species of the genus , for which the name sp. nov. is proposed. The type strain is TS8 (=DSM 114830=NCIMB 15451).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.006361
2024-05-15
2024-05-29
Loading full text...

Full text loading...

References

  1. Yan S, Yu M, Wang Y, Shen C, Zhang X-H. Catenovulum agarivorans gen. nov., sp. nov., a peritrichously flagellated, chain-forming, agar-hydrolysing gammaproteobacterium from seawater. Int J Syst Evol Microbiol 2011; 61:2866–2873 [View Article] [PubMed]
    [Google Scholar]
  2. Li DQ, Zhou YX, Liu T, Chen GJ, Du ZJ. Catenovulum maritimus sp. nov., a novel agarolytic gammaproteobacterium isolated from the marine alga Porphyra yezoensis Ueda (AST58-103), and emended description of the genus Catenovulum. Antonie van Leeuwenhoek 2015; 108:427–434 [View Article] [PubMed]
    [Google Scholar]
  3. Shi MJ, Du WJ, Wang C, Chen GJ, Du ZJ. Catenovulum sediminis sp. nov., isolated from coastal sediment. Int J Syst Evol Microbiol 2017; 67:3894–3898 [View Article] [PubMed]
    [Google Scholar]
  4. Yun EJ, Yu S, Kim KH. Current knowledge on agarolytic enzymes and the industrial potential of agar-derived sugars. Appl Microbiol Biotechnol 2017; 101:5581–5589 [View Article] [PubMed]
    [Google Scholar]
  5. Schoch T, Höfele F, Odeh H, Winter L-M, Stöferle S et al. Reclassification of Clostridium aurantibutyricum Hellinger 1944 and Clostridium roseum (ex McCoy and McClung 1935) Cato et al. 1988. Int J Syst Evol Microbiol 2022; 72:005589 [View Article]
    [Google Scholar]
  6. Chen S, Zhou Y, Chen Y, Gu J. fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics 2018; 34:i884–i890 [View Article] [PubMed]
    [Google Scholar]
  7. Wick RR, Menzel P. Filtlong. v0.2.1 Edition github; 2021
    [Google Scholar]
  8. Wick RR, Judd LM, Gorrie CL, Holt KE. Unicycler: resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol 2017; 13:e1005595 [View Article] [PubMed]
    [Google Scholar]
  9. Wick RR, Holt KE. Polypolish: short-read polishing of long-read bacterial genome assemblies. PLoS Comput Biol 2022; 18:e1009802 [View Article] [PubMed]
    [Google Scholar]
  10. Zimin AV, Marçais G, Puiu D, Roberts M, Salzberg SL et al. The MaSuRCA genome assembler. Bioinformatics 2013; 29:2669–2677 [View Article] [PubMed]
    [Google Scholar]
  11. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol Biol Evol 2018; 35:1547–1549 [View Article] [PubMed]
    [Google Scholar]
  12. Meier-Kolthoff JP, Göker M. TYGS is an automated high-throughput platform for state-of-the-art genome-based taxonomy. Nat Commun 2019; 10:2182 [View Article] [PubMed]
    [Google Scholar]
  13. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence aalysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [View Article]
    [Google Scholar]
  14. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:1–14 [View Article] [PubMed]
    [Google Scholar]
  15. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007; 57:81–91 [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.006361
Loading
/content/journal/ijsem/10.1099/ijsem.0.006361
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error