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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 5

sp. nov., isolated from human stomach No Access

Abstract

A Gram-negative, facultative anaerobic, non-motile and rod-shaped bacterium, designated 10c7w1, was isolated from a human gastrointestinal tract. Colonies on agar plates were small, circular, smooth and beige. The optimal growth conditions were determined to be 37 °C, pH 7.0–7.5 and 0 % (w/v) NaCl. Comparative analysis of complete 16S rRNA gene sequences revealed that strain 10c7w1 showed the highest sequence similarity of 95.8 % to MCCC 1A01410, followed by (95.2 %) JCM 11629. The average amino acid identity values between 10c7w1 and MCCC 1A01410 and JCM 11629 were above 60 % (71.4 and 69.5 %). The average nucleotide identity values between strain 10c7w1 and MCCC 1A01410 and JCM 11629 were 76.9 and 72.5 %, respectively. The dominant fatty acids (≥10 %) were straight chain ones, with summed feature 3 (C 7/C 6), summed feature 8 (C 7/C 6) and C being the most abundant. Q-8 was the only respiratory quinone. The major polar lipids of strain 10c7w1 were phosphatidylethanolamine, diphosphatidylglycerol and unknown lipids. The DNA G+C content of strain 10c7w1 was 63.6 mol%. On the basis of phylogenetic, phenotypic and chemotaxonomic data, strain 10c7w1 is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 10c7w1 (=MCCC 1H01399=KCTC 92200).

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Keyword(s): 16S rRNA gene , Ottowia cancrivicina and polyphasic taxonomy
Funding
This study was supported by the:
  • Incubation Projects of Weihai Municipal Hospital (Award FH-2021-XZ02)
    • Principle Award Recipient: Ming-YiWang
  • Shandong Provincial Natural Science Foundation (Award ZR2021MH409)
    • Principle Award Recipient: Zong-JunDu
© 2024 The Authors
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2024-05-10
2025-05-21
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Ottowia cancrivicina sp. nov., isolated from human stomach
Int J Syst Evol Microbiol 74, 006360 (2024); https://doi.org/10.1099/ijsem.0.006360
/content/journal/ijsem/10.1099/ijsem.0.006360
/content/journal/ijsem/10.1099/ijsem.0.006360
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