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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 2

sp. nov. and sp. nov., isolated from the gut of honeybee () No Access

Abstract

Four lactic acid bacteria, designated F690, F697, F790 and F769-2, were isolated from the gut of honeybee (). Results of 16S rRNA gene sequence analysis indicated that strains F690 and F697 were phylogenetically related to the type strains of , , and , having 99.1–99.6 % 16S rRNA gene sequence similarities; and that strains F790 and F769-2 were most closely related to the type strain of , having 99.2–99.3 % 16S rRNA gene sequence similarities. The phylogenies based on concatenated , , , , , and sequences and based on whole genome sequences were identical to that based on 16S rRNA gene sequences. Strains F690 and F697 exhibited the highest average nucleotide identity (ANI; 92.1–93.2 %), digital DNA–DNA hybridization (dDDH; 50–50.1 %) and average amino acid identity (AAI; 94.9–95.1 %) values with Hma2N. Strains F790 and F769-2 had the highest ANI (93.1–94 %), dDDH (54.4 %) and AAI (94.4–94.7 %) values with Hma8N. Based upon the data obtained in the present study, two novel species, sp. nov. and sp. nov., are proposed and the type strains are F690 (=JCM 36259=CCTCC AB 2023131) and F790 (=JCM 36260=CCTCC AB 2023132), respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): honeybee , lactic acid bacteria , Lactobacillus and novel species
Funding
This study was supported by the:
  • “Characteristic Probiotics and New Fermented Food” Team in Northeast Agricultural University (Award 50940912, Harbin, China)
    • Principle Award Recipient: ChunTao Gu
  • National Natural Science Foundation of China (Award no. 31471594)
    • Principle Award Recipient: ChunTao Gu
© 2024 The Authors
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2024-02-29
2025-01-15
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Lactobacillus juensis sp. nov. and Lactobacillus rizhaonensis sp. nov., isolated from the gut of honeybee (Apis mellifera)
Int J Syst Evol Microbiol 74, 006285 (2024); https://doi.org/10.1099/ijsem.0.006285
/content/journal/ijsem/10.1099/ijsem.0.006285
/content/journal/ijsem/10.1099/ijsem.0.006285
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