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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 1

gen. nov., sp. nov., a novel member of family isolated from activated sludge collected in Shenzhen, PR China No Access

Abstract

A Gram-stain-negative, strictly aerobic and filamentous bacterial strain, designated as DQS-5, was isolated from the activated sludge of a municipal sewage treatment plant in Shenzhen, PR China. Optimal growth was observed at 28 °C and pH 7.5. Catalase and oxidase activities were detected. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DQS-5 was most closely related to the genera and (91.0–93.4 % and 92.5 % 16S rRNA gene sequence similarity, respectively) and was close to the member of the family . The complete genome sequence of strain DQS-5 contains 5 653 844 bp and 57.3 mol% G+C. The average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values between the genome of strain DQS-5 and those of its close relatives were 75.9–77.2, 19.0–20.3 and 57.2–61.8 %, respectively. Chemotaxonomic analysis of strain DQS-5 indicated that the sole respiratory quinone was ubiquinone-8, the predominant cellular fatty acids were C and summed feature 3 (C ω7 and/or C ω6), and the major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, aminophospholipid and aminolipid. The phylogenetic, genotypic, phenotypic and chemotaxonomic data demonstrate that strain DQS-5 represents a novel species in a novel genus within the family , for which the name gen. nov., sp. nov., is proposed. Strain DQS-5 (=KCTC 92788=CCTCC AB 2022320) is the type and only strain of .

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  • Accepted:
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Keyword(s): activated sludge , novel genus and Parachitinimonas caeni
Funding
This study was supported by the:
  • Shandong Provincial Education Science Plan (Award Q20194303)
    • Principle Award Recipient: HanChen
  • Shenzhen Municipal Key Laboratory of Neuropsychiatric Modulation, Chinese Academy of Sciences (Award Award XDA23040401)
    • Principle Award Recipient: DongruQiu
  • National Key R&D Program of China (Award 2019YFA0905500)
    • Principle Award Recipient: DongruQiu
© 2024 The Authors
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2024-01-29
2024-12-14
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Parachitinimonas caeni gen. nov., sp. nov., a novel member of family Burkholderiaceae isolated from activated sludge collected in Shenzhen, PR China
Int J Syst Evol Microbiol 74, 006249 (2024); https://doi.org/10.1099/ijsem.0.006249
/content/journal/ijsem/10.1099/ijsem.0.006249
/content/journal/ijsem/10.1099/ijsem.0.006249
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