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Volume 74, Issue 1

gen. nov., sp. nov., a new member of the family , isolated from a marine sediment of the South China Sea No Access

Abstract

A Gram-stain-negative, catalase-positive and oxidase-positive, nonmotile, aerobic, light yellow, spherical-shaped bacterial strain with no flagella, designated strain YIM 152171, was isolated from sediment of the South China Sea. Colonies were smooth and convex, light yellow and circular, and 1.0–1.5×1.0–1.5 µm in cell diameter after 7 days of incubation at 28°C on YIM38 media supplemented with sea salt. Colonies could grow at 20–45°C (optimum 28–35°C) and pH 6.0–11.0 (optimum, pH 7.0–9.0), and they could proliferate in the salinity range of 0–6.0 % (w/v) NaCl. The major cellular fatty acids were summed feature 8 (C 7/C ω6), C ω7 11-methyl, C, C ω11, C ω5, C ω6 and C ω5. The respiratory quinone was ubiquinone 10, and the polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol mannoside, one unidentified phospholipid and one unidentified aminolipid. Phylogenetic analyses based on the 16S rRNA gene sequences placed strain YIM 152171 within the order in a distinct lineage that also included the genus . The 16S rRNA gene sequence similarities of YIM 152171 to those of , and were 92.17, 89.25 and 88.91 %, respectively. The assembled draft genome of strain YIM 152171 had 136 contigs with an N50 value of 134704 nt, a total length of 3 001 346 bp and a G+C content of 70.27 mol%. The phylogenetic, phenotypic and chemotaxonomic data showed that strain YIM 152171 (=MCCC 1K08488=KCTC 92884) represents a type of novel species and genus for which we propose the name gen. nov., sp. nov.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genome , marine sediment , Marinimicrococcus flavescens , South China Sea and taxonomy
Funding
This study was supported by the:
  • the Government Project of Yunnan Province (Award YNWR-QNBJ-2018-085)
    • Principle Award Recipient: YiJiang
  • Major research project of Guangxi for science and technology (Award AA18242026)
    • Principle Award Recipient: YiJiang
  • National Natural Science Foundation of China (Award No.32060001)
    • Principle Award Recipient: YiJiang
© 2024 The Authors
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2024-12-07
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/content/journal/ijsem/10.1099/ijsem.0.006241
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Marinimicrococcus flavescens gen. nov., sp. nov., a new member of the family Geminicoccaceae, isolated from a marine sediment of the South China Sea
Int J Syst Evol Microbiol 74, 006241 (2024); https://doi.org/10.1099/ijsem.0.006241
/content/journal/ijsem/10.1099/ijsem.0.006241
/content/journal/ijsem/10.1099/ijsem.0.006241
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