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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 1

sp. nov., a heavy-metal-tolerant bacterium isolated from garden soil No Access

Abstract

A previously undescribed, heavy-metal-tolerant, motile, Gram-negative bacterium, designated strain SK50-23, was characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SK50-23 was closely related to LMG 26467 and the non-phototrophic ‘’ NS23 (98.1 and 97.3 % 16S rRNA gene sequence similarity, respectively). Strain SK50-23 possessed a circular genome of 5.86 Mb, with a DNA G+C content of 61.9 mol%. Digital DNA–DNA hybridization showed 20.8–21.6 % similarity between strain SK50-23 and related species. In addition, the whole-genome average nucleotide identity values between strain SK50-23 and related species ranged from 75.1 to 83.5 %. The major cellular fatty acid identified in strain SK50-23 was Cω7, and the main isoprenoid quinone present was ubiquinone Q-10. Strain SK50-23 could be assigned to the genus with the species name sp. nov. based on morphological, chemotaxonomic and genome-based taxonomic characteristics, and 16S rRNA gene-based phylogenetic characteristics. The type strain of the proposed novel species is SK50-23 (=NBRC 108825=CGMCC No. 1.12037).

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  • Accepted:
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Keyword(s): novel species , Tardiphaga alba and Tardiphaga genome
Funding
This study was supported by the:
  • National Science Foundation of China (Award 41963008)
    • Principle Award Recipient: ZhihuaBao
  • National Science Foundation of China (Award 32261143732)
    • Principle Award Recipient: ZhihuaBao
© 2024 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.006238
2024-01-10
2025-01-26
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/content/journal/ijsem/10.1099/ijsem.0.006238
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Tardiphaga alba sp. nov., a heavy-metal-tolerant bacterium isolated from garden soil
Int J Syst Evol Microbiol 74, 006238 (2024); https://doi.org/10.1099/ijsem.0.006238
/content/journal/ijsem/10.1099/ijsem.0.006238
/content/journal/ijsem/10.1099/ijsem.0.006238
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