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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 1

sp. nov., producing the glycopeptide antibiotic A477 No Access

Abstract

In 1973, Eli Lilly and Company described the filamentous actinomycete producing the glycopeptide antibiotic A477 as an species on the basis of its morphological and physiological features and deposited it as NRRL 3884. In this paper, we report that the phylogenetic analysis based on the 16S rRNA gene sequence and the whole genome phylogenomic study indicate that NRRL 3884 forms a distinct monophyletic line within the genus , being most closely related to NBRC 14524 [99.6 % 16S rRNA gene similarity, 89.4 % average nucleotide identity (ANI), 46.0 % digital DNA–DNA hybridization (dDDH)] and NBRC 13996 (98.8 % 16S rRNA gene similarity, 89.0 % ANI, 47.0 % dDDH). NRRL 3884 forms an extensively branched, non-fragmented vegetative mycelium; either sterile aerial hyphae or regular subglobose sporangia are formed depending on cultivation conditions. The cell wall contains -2,6-diaminopimelic acid and 2,6-diamino-3-hydroxypimelic acid and the diagnostic sugars are glucose, mannose and ribose with a minor amount of rhamnose. The predominant menaquinone (MK) is MK-9(H), with minor amounts of MK-9(H), MK-9(H) and MK-9(H). Mycolic acids are absent. The diagnostic phospholipids are diphosphatidylglycerol and phosphatidylethanolamine. The major cellular fatty acids are anteiso-C, iso-C and iso-C, with moderate amounts of anteiso-C and iso-C. The genomic G+C content is 71.5 mol%. Significant differences in the genomic, morphological, chemotaxonomic and biochemical data between NRRL 3884 and the two most closely related type strains clearly demonstrate that NRRL 3884 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NRRL 3884 (=DSM 116196).

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  • Accepted:
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Keyword(s): A477 , actinomycetes , Actinoplanes oblitus sp. nov. , Actinoplanes sp. NRRL 3884T and glycopeptide antibiotics
Funding
This study was supported by the:
  • Fondo Ateneo per la Ricerca (Award Fondo Ateneo per la Ricerca (FAR) 2020-2021)
    • Principle Award Recipient: FlaviaMarinelli
© 2024 The Authors
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Actinoplanes oblitus sp. nov., producing the glycopeptide antibiotic A477
Int J Syst Evol Microbiol 74, 006225 (2024); https://doi.org/10.1099/ijsem.0.006225
/content/journal/ijsem/10.1099/ijsem.0.006225
/content/journal/ijsem/10.1099/ijsem.0.006225
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