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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 1

sp. nov., a bacterium isolated from rhizosphere soil of rice No Access

Abstract

A Gram-negative and rod-shaped bacterium, designated C340-1, was isolated and screened from paddy soil in Zhongshan County, Guangxi Province, PR China. This strain grew at 20–42 °C (optimum, 37 °C), pH 5.0–9.0 (optimum, pH 7.0) and 0–4 % (w/v) NaCl (optimum, 0–1 %) on Reasoner's 2A medium. The strain could fix atmospheric nitrogen and acetylene reduction activity was recorded up to 120.26 nmol ethylene h (mg protein). Q-10 was the only isoprenoid quinone component; phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid and an unidentified polar lipid were the major polar lipids. Summed feature 8 (C 7 and/or C 6) and summed feature 3 (C 7 and/or C 6) were the primary cellular fatty acids. The genome of strain C340-1 was 6.18 Mb, and the G+C content was 69.0 mol%. Phylogenetic tree analysis based on 16S rRNA gene and 92 core genes showed that strain C340-1 was closely related to and clustered with the type strains JCM 1224, Az39, Sp245 and JCM 17639. The average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA–DNA hybridization (dDDH) values between strain C340-1 and the closely related type strains mentioned above were significantly lower than the threshold values for species classification (95–96 %, 95–96 % and 70 %, respectively). Based on phylogenetic, genomic, phenotypic, physiological and biochemical data, we have reason to believe that C340-1 represents a new species of the genus , for which the name sp. nov. is proposed. The type strain is C340-1(=CCTCC AB 2023105=KCTC 8126).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Azospirillum; polyphasic taxonomy; N2 fixation , indole-3-acetic acid (IAA) , paddy soil and plant growth-promoting bacteria
Funding
This study was supported by the:
  • Jiangsu Provincial Agricultural Science and Technology Independent Innovation Fund (Award CX(20)2003)
    • Principle Award Recipient: ZubinXie
  • National Science and Technology Infrastructure Program (Award 2015FY110700)
    • Principle Award Recipient: ZubinXie
  • Innovative Research Group Project of the National Natural Science Foundation of China (Award 42177333)
    • Principle Award Recipient: ZubinXie
© 2024 The Authors
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Azospirillum isscasi sp. nov., a bacterium isolated from rhizosphere soil of rice
Int J Syst Evol Microbiol 74, 006218 (2024); https://doi.org/10.1099/ijsem.0.006218
/content/journal/ijsem/10.1099/ijsem.0.006218
/content/journal/ijsem/10.1099/ijsem.0.006218
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