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Volume 74, Issue 1

sp. nov., isolated from seleniferous soil No Access

Abstract

Two Gram-stain-negative, facultative anaerobic, rod-shaped bacterial strains, S171 and S2-9, were isolated from seleniferous soil in China. Comprehensive phylogenetic analyses based on 16S rRNA genes, multilocus sequences and whole genome sequences indicated that the two strains belonged to the genus . The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values of strains S171 and S2-9 with the closest relative NCTC 10786 were 83.6–83.7% and 27.7–27.8 %, respectively, which were below the species cutoff values. The ANI and dDDH values between the two strains were 97.9% and 84.8 %, respectively. The biochemical characteristics revealed that selenite tolerance, HS and indole production, arginine dihydrolase, ornithine decarboxylase, as well as acid production from carbon sources such as -sorbitol and arbutin are distinctive features of the two strains. Based on these results, strain S171 and strain S2-9 represent a novel species of the genus , for which the name sp. nov. is proposed, with strain S171 (=GDMCC 1.3637=JCM 35851) as the type strain. The genome size of strain S171 was 4.92 Mb with a G+C content of 52.6 mol%. The genome size of strain S2-9 was 4.89 Mb with a G+C content of 52.6 mol%.

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  • Accepted:
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Keyword(s): Citrobacter , seleniferous soil and selenium-tolerant bacterium
Funding
This study was supported by the:
  • Special Fund for Key Science & Technology Program in Yunnan Province (Award No. 202202AE090029)
    • Principle Award Recipient: GuoYanbin
  • Special Fund for Key Science & Technology Program in Xinjiang Province (Award No. 2022B02053-1)
    • Principle Award Recipient: GuoYanbin
  • Special Fund for Agro-scientific Research in the Public Interest (Award 201303106)
    • Principle Award Recipient: YanbinGuo
© 2024 The Authors
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Citrobacter enshiensis sp. nov., isolated from seleniferous soil
Int J Syst Evol Microbiol 74, 006154 (2024); https://doi.org/10.1099/ijsem.0.006154
/content/journal/ijsem/10.1099/ijsem.0.006154
/content/journal/ijsem/10.1099/ijsem.0.006154
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