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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 7

sp. nov., a spore-forming lactic acid bacterium isolated from tree bark in Thailand No Access

Abstract

A Gram-stain-positive, facultatively anaerobic and endospore-forming rod-shaped bacterium, designed strain CPB3-1, was isolated from tree bark. This homofermentative strain produced -lactic acid from glucose. It grew at 20–45 °C, pH 4.0–9.5 and in 0-3.0 % (w/v) NaCl. It contained -diaminopimelic acid in cell-wall peptidoglycan and had menaquinone with seven isoprene units (MK-7) as the predominant component. The major fatty acid was anteiso-C. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipid and an unknown lipid. Based on the results of 16S rRNA gene sequence analysis, strain CPB3-1 belonged to the genus and was closely related to DSM 11701 and BK117-1 (both 96.7 % similarity), NRIC 1133 and DSM 11697 (both 96.6 % similarity), and MK21-7, DSM 442, BK92 and GD201205 (all 95.8–96.5 % similarity). The draft genome of strain CPB3-1 contained 2 930 919 bps with 3117 coding genes. The DNA G+C content was 45.1 mol%. The digital DNA–DNA hybridization values between strain CPB3-1 and closely related type strains were 19.2–24.0 %. The average nucleotide identity (84.0–87.6 %) and average amino acid identity (66.5–76.3 %) values were lower than the cut-off values for species delineation. Strain CPB3-1 was clearly distinguished from related species based on its phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequence similarity and the results of draft genome analysis. Therefore, the strain represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CPB3-1 (=JCM 35082=TISTR 10004).

  • Received:
  • Accepted:
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Keyword(s): lactic acid bacteria , polyphasic taxonomy , spore-forming bacteria , Sporolactobacillus mangiferae and tree bark
Funding
This study was supported by the:
  • the Grant of Faculty of Pharmaceutical Sciences, Chulalongkorn University (Award Phar2565-RG002)
    • Principle Award Recipient: SomboonTanasupawat
© 2023 The Authors
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2023-07-26
2025-03-23
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Sporolactobacillus mangiferae sp. nov., a spore-forming lactic acid bacterium isolated from tree bark in Thailand
Int J Syst Evol Microbiol 73, 005993 (2023); https://doi.org/10.1099/ijsem.0.005993
/content/journal/ijsem/10.1099/ijsem.0.005993
/content/journal/ijsem/10.1099/ijsem.0.005993
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