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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 7

sp. nov., isolated from desert soil No Access

Abstract

Two Gram-stain-negative strains, designated as SYSU D00286 and SYSU D00782, were isolated from a sand sample collected from the Kumtag Desert in Xinjiang, north-west China. Cells were aerobic, non-motile and positive for both oxidase and catalase. Growth occurred at 4–37 °C (optimum, 28–30 °C), pH 6.0–7.0 (optimum, pH 7.0) and NaCl concentration of 0–1.5 % (w/v; optimum, 0%). Growth was observed on Reasoner’s 2A agar and nutrient agar, but not on Luria–Bertani agar and trypticase soy agar. The polar lipids were identified as diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, three unidentified aminolipids, one unidentified glycolipid and two unidentified phospholipids. The major respiratory quinone was ubiquinone-10 and the major fatty acids (>10 %) were C and summed feature 8 (C 7 and/or C 6). The 16S rRNA gene sequence similarity between strains SYSU D00286 and SYSU D00782 was 100%, and their average nucleotide identity (ANI), average amino acid identity and (AAI) digital DNA–DNA hybridization (dDDH) values were all 100.0 %. Phylogenetic analysis indicated that these two strains belong to the same species of the genus and show the highest sequence similarity to KCTC 72461 (98.2 %) and CCTCC AA 208029 (97.5 %). The ANI, AAI and dDDH values between SYSU D00286 (as well as SYSU D00782) and the other five type strains were all less than or equal to 83.2, 80.1 and 23.6 %, respectively. Based on their phylogenetic, phenotypic and chemotaxonomical features, strains SYSU D00286 and SYSU D00782 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SYSU D00286 (=MCCC 1K04981=CGMCC 1.8626=KCTC 82271).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Kumtag Desert , polyphasic taxonomy and Rubellimicrobium arenae sp. nov.
Funding
This study was supported by the:
  • the Third Xinjiang Scientific Expedition Program (Award 2022xjkk1204)
    • Principle Award Recipient: LeiDong
  • National Natural Science Foundation of China (Award 32270076)
    • Principle Award Recipient: LeiDong
  • National Natural Science Foundation of China (Award 32061143043)
    • Principle Award Recipient: Wen-JunLi
© 2023 Sun Yat-Sen University
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2023-07-25
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Rubellimicrobium arenae sp. nov., isolated from desert soil
Int J Syst Evol Microbiol 73, 005990 (2023); https://doi.org/10.1099/ijsem.0.005990
/content/journal/ijsem/10.1099/ijsem.0.005990
/content/journal/ijsem/10.1099/ijsem.0.005990
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