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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 7

sp. nov. isolated from brackish water of the Tagus estuary in Alcochete, Portugal No Access

Abstract

An isolation effort focused on sporogenous from the Tagus estuary in Alcochete, Portugal, yielded a novel actinomycetal strain, designated MTZ3.1, which was subjected to a polyphasic taxonomic study. MTZ3.1 is characterised by morphology typical of members of the genus , with light beige coloured substrate mycelium, which does not release pigments to the culture medium and with helicoidal aerial hyphae that differentiate into spores with a light-grey colour. The phylogeny of MTZ3.1, based on the full 16S rRNA gene sequence, indicated that its closest relatives were OF1 (98.48 %), KK1-2 (98.41 %), JCM 4342 (98.34 %), NBRC 15454 (98.34 %) and NRBC 13444 (98.34 %). Moreover, average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA–DNA hybridisation (dDDH) are below the species cutoff values (ANI 67.70 and 68.35 %, AAI 77.06 and 76.71 % and dDDH 22.10 and 21.50 % for OF1 and KK1-2, respectively). Whole genome sequencing revealed that MTZ3.1 has a genome of 5 644 485 bp with a DNA G+C content of 71.29 mol% and 5044 coding sequences. Physiologically, MTZ3.1 is strictly aerobic, able to grow at 15–37 °C, optimally at 25 °C and between pH5 and 8 and showed high salinity tolerance, growing with 0–10 %(w/v) NaCl. Major cellular fatty acids are C, iso-C, anteiso-C and iso-C. Furthermore, it was able to utilise a variety of nitrogen and carbon sources. Antimicrobial screening indicated that MTZ3.1 has potent anti- activity. On the basis of the polyphasic data, MTZ3.1 is proposed to represent a novel species, sp. nov. (= CECT 30416 = DSM 114037=LMG 32463).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Actinomycetota , brackish water , novel taxa , Streptomyces and Tagus river estuary
Funding
This study was supported by the:
  • Fonds National de la Recherche Luxembourg (Award ARF scheme, Ref. 14583934)
    • Principle Award Recipient: DominikaKlimek
  • Fundação para a Ciência e a Tecnologia (Award SFRH/BD/145576/2019)
    • Principle Award Recipient: JoséDiogo Neves dos Santos
  • Fundação para a Ciência e a Tecnologia (Award UIDP/04423/2020)
    • Principle Award Recipient: OlgaMaria Lage
© 2023 The Authors
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2023-07-24
2025-04-23
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Streptomyces meridianus sp. nov. isolated from brackish water of the Tagus estuary in Alcochete, Portugal
Int J Syst Evol Microbiol 73, 005987 (2023); https://doi.org/10.1099/ijsem.0.005987
/content/journal/ijsem/10.1099/ijsem.0.005987
/content/journal/ijsem/10.1099/ijsem.0.005987
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