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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 7

sp. nov. isolated from Florida manatees () No Access

Abstract

Four novel strains of a member of the genus (Oakley, Holly, Lorelei and Ariel) were isolated from skin of Florida manatees (). These strains were phenotypically and genetically characterized and compared with the known species of the genera (.) (.), (.) (.) and (.). All the strains produced acid from glucose but did not hydrolyze arginine or urea. All were propagated in ambient air supplemented with 5±1 % CO at 35–37 °C using SP4-Z, Columbia and brain–heart infusion medium. Colonies on solid medium showed a typical fried-egg appearance and transmission electron microscopy revealed a typical mycoplasma-like cellular morphology. The results of phylogenetic analyses based on partial 16S rRNA, , and gene sequences and the whole proteome data indicated that the novel species is a unique species but phylogenetically closely related to , and ''. The average nucleotide identity and digital DNA–DNA hybridization values between strain Oakley and the closely related species were significantly lower than the accepted thresholds for describing novel prokaryotic species at the genomic level. On the basis of the genomic, phenotypic and phylogenetic properties, the novel strains represent a novel species of the genus , for which the name sp. nov. with the type strain Oakley (=NCTC 14352 =DSM 110686) is proposed. The genomic DNA G+C content and complete draft genome size for the type strain are 38.35 % and 1 873 856 bp, respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA , Acholeplasma , manatee , marine mammals , Paracholeplasma and phylogeny
© 2023 The Authors
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2023-07-25
2024-12-14
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Paracholeplasma manati sp. nov. isolated from Florida manatees (Trichechus manatus latirostris)
Int J Syst Evol Microbiol 73, 005973 (2023); https://doi.org/10.1099/ijsem.0.005973
/content/journal/ijsem/10.1099/ijsem.0.005973
/content/journal/ijsem/10.1099/ijsem.0.005973
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