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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 7

gen. nov. sp. nov., isolated from a human clinical sample Open Access

Abstract

The diversity of bacteria associated with biopsy material obtained from patients with colorectal cancer was investigated using culture techniques. A novel bacterium, strain CC70A, was isolated by diluting a sample of homogenized tissue in anaerobic medium, and then plating to yield a pure culture. Strain CC70A was a Gram-positive, strictly anaerobic, motile, rod-shaped bacterium. Formate, but not acetate, was a fermentative end-product from growth in peptone–yeast extract and peptone–yeast–glucose broth. The G+C content of DNA from strain CC70A was 34.9 mol%. 16S rRNA gene sequence analysis revealed that the isolate was part of the phylum . The closest described relatives of strain CC70A were (93.3 %) and (93.3 and 91.9% sequence similarity across 16S rRNA gene, respectively). According to the data obtained in this work, strain CC70A represents a novel bacterium belonging to a new genus for which the name gen. nov., sp. nov. is proposed. The type strain for our described novel species is CC70A (=DSM 27931= JCM 30568).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA , average amino acid identity , Cellulosilyticum , genome and taxonomy
Funding
This study was supported by the:
  • Cancer Research UK (Award A27140)
    • Principle Award Recipient: EmmaAllen-Vercoe
  • Canadian Cancer Society Research Institute (Award i2I17-1)
    • Principle Award Recipient: EmmaAllen-Vercoe
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-07-12
2025-05-19
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Holtiella tumoricola gen. nov. sp. nov., isolated from a human clinical sample
Int J Syst Evol Microbiol 73, 005958 (2023); https://doi.org/10.1099/ijsem.0.005958
/content/journal/ijsem/10.1099/ijsem.0.005958
/content/journal/ijsem/10.1099/ijsem.0.005958
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