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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 7

sp. nov., isolated from marine beach sediment No Access

Abstract

A novel actinomycetal strain, designated M600PL45_2, was isolated from marine sediments obtained from Ingleses beach, Porto, on the Northern Coast of Portugal and was subjected to a polyphasic taxonomic characterisation study. The here described Gram-reaction-positive strain is characterised by the production of a brown pigment in both solid and liquid medium and forms typical helical hyphae that differentiate into smooth spores. The results of a phylogenetic analysis based on the 16S rRNA gene sequence indicated that M600PL45_2 has a high similarity to two members of the genus , ASO4wet (98.51 %) and NEAU ZJC8 (98.44 %). The genome of M600PL45_2 has a size of 6 695 159 bp, a DNA G+C content of 70.71 mol% and 5538 coding sequences. M600PL45_2 grows at 15–37 °C and with a maximal growth rate between 25 °C and 30 °C. Growth at pH 6.0 to 9.0 with the optimal range between 6.0 and 7.5 was observed. M600PL45_2 showed a high salinity tolerance, growing with 0–10 % (w/v) NaCl, with best growth with 1–3% (w/v) NaCl. Major cellular fatty acids are iso-C (25.03 %), anteiso-C (17.70) and iso-C (26.90 %). The novel isolate was able to grow in media containing a variety of nitrogen and carbon sources. An antimicrobial activity screening indicated that an extract of M600PL45_2 has inhibitory activity against . On the basis of the polyphasic data, M600PL45_2 (= CECT 30365 = DSM 114036) is introduced as the type strain of a novel species, that we named sp. nov.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Actinomycetota , antimicrobial activity , genome mining , novel taxa and Streptomyces
Funding
This study was supported by the:
  • German DFG Collaborative Research Centre AquaDiva (Award CRC 1076)
    • Principle Award Recipient: Not Applicable
  • Fundação para a Ciência e a Tecnologia (Award SFRH/BD/145577/2019)
    • Principle Award Recipient: Inês Rosado Vitorino
  • Fundação para a Ciência e a Tecnologia (Award SFRH/BD/145576/2019)
    • Principle Award Recipient: José Diogo Neves dos Santos
  • Fundação para a Ciência e a Tecnologia (Award UIDP/04423/2020)
    • Principle Award Recipient: Olga Maria Lage
  • Fundação para a Ciência e a Tecnologia (Award UIDB/04423/2020)
    • Principle Award Recipient: Olga Maria Lage
© 2023 The Authors
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2023-07-25
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Streptomyces marispadix sp. nov., isolated from marine beach sediment
Int J Syst Evol Microbiol 73, 005956 (2023); https://doi.org/10.1099/ijsem.0.005956
/content/journal/ijsem/10.1099/ijsem.0.005956
/content/journal/ijsem/10.1099/ijsem.0.005956
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