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Volume 73, Issue 6

gen. nov. sp. nov., isolated from mangrove soil and insight into its ecological distribution and metabolic potential No Access

Abstract

A Gram-stain-negative, facultatively anaerobic, motile and rod-shaped bacterium, designated as 6D33, was isolated from mangrove soil. Growth was found to occur at 15–32 °C (optimum, 28 °C), at pH 6–9 (optimum, pH 7) and in 0–3 % NaCl (optimum, 1 %, w/v). The results of 16S rRNA gene-based analysis showed that strain 6D33 belonged to the family , sharing 93.1–94.4 % identity with its close neighbours within the genus . The phylogenomic results indicated that strain 6D33 formed an independent branch distinct from type strains of the genus . The overall genome relatedness indices of digital DNA–DNA hybridization, average nucleotide identity and amino acid identity values showed that strain 6D33 represents a novel species of a novel genus. The results of chemotaxonomic characterization indicated that the major cellular fatty acids of strain 6D33 were summed feature 9 (C 10-methyl and/or iso-C 9), summed feature 3 (C 6 and/or C 7) and iso-C; the polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid and three unidentified lipids; the only respiratory quinone was ubiquinone-10. The genomic size and DNA G+C contents were 3.59 Mbp and 60.84 mol%, respectively. The 16S rRNA gene sequence reads abundance profiles revealed that the rare taxon is prevalent in marine environments, especially in sediments. Genome-scale metabolic reconstruction of strain 6D33 revealed a heterotrophic lifestyle and many pathways responsible for the degradation of aromatic compounds, suggesting application potential in aromatic hydrocarbon removal. Based on its genotypic and phenotypic characteristics, strain 6D33 is concluded to represent a novel species of the novel genus in the family , for which the name gen. nov. sp. nov. is proposed. The type strain of the type species is 6D33 (=GDMCC 1.1959=KCTC 82335).

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  • Accepted:
  • Published Online:
Keyword(s): ecological distribution , Gimibacter soli , metabolic potential and polyphasic taxonomy
Funding
This study was supported by the:
  • GDAS’ Project of Science and Technology Development (Award 2022GDASZH-2022010201)
    • Principle Award Recipient: NotApplicable
  • National Natural Science Foundation of China (Award 32070115)
    • Principle Award Recipient: NotApplicable
  • Guangdong Special Support Program (Award 2021JC06N628)
    • Principle Award Recipient: HonghuiZhu
© 2023 The Authors
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2024-10-11
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Gimibacter soli gen. nov. sp. nov., isolated from mangrove soil and insight into its ecological distribution and metabolic potential
Int J Syst Evol Microbiol 73, 005953 (2023); https://doi.org/10.1099/ijsem.0.005953
/content/journal/ijsem/10.1099/ijsem.0.005953
/content/journal/ijsem/10.1099/ijsem.0.005953
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