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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 7

sp. nov., a novel actinobacterium isolated from gut of larvae of No Access

Abstract

A novel actinobacterium strain, designated CFWR-12, was isolated from the larval gut of grown at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea, and its taxonomic position was evaluated. Strain CFWR-12 was aerobic, Gram-stain-positive and non-motile. Growth occurred at 10–40 °C, pH 6.0–9.0 and 0–4 % (w/v) NaCl, with optimal growth at 28–30 °C, pH 7.0 and in the absence of NaCl. Strain CFWR-12 showed high 16S rRNA gene sequence similarity to KACC 19306 (99.0 %) and FW100M-8 (97.9 %). The genome sequence of strain CFWR-12 was 4.01 Mb in size with a high G+C content of 71.2 mol%. The values of average nucleotide identity and digital DNA–DNA hybridization between strain CFWR-12 and KACC 19306 were 89.8 and 39.1 %, respectively, which were the highest among the closely related species. The predominant cellular fatty acids (>10 %) were iso-C, anteiso-C and anteiso-C, and the major respiratory quinones (>10 %) were MK-11 and MK-12. The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid and an unidentified lipid while the peptidoglycan type was identified to be B1. Data based on chemotaxonomic, phylogenetic, phenotypic and genomic evidence demonstrated that strain CFWR-12 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain CFWR-12 (=KACC 19307= NBRC 113047).

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Keyword(s): Agromyces larvae sp. nov. , CFWR-12T , larvae gut and Protaetia brevitarsis seulensis
© 2023 The Authors
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2023-07-06
2025-11-15

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References

  1. Gledhill WE, Casida LE. Predominant catalase-negative soil bacteria. III. Agromyces, gen. n., microorganisms intermediary to Actinomyces and Nocardia. Appl Microbiol 1969; 18:340–349 [View Article] [PubMed]
     [Google Scholar]
  2. Parte AC, Sardà Carbasse J, Meier-Kolthoff JP, Reimer LC, Göker M. List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. Int J Syst Evol Microbiol 2020; 70:5607–5612 [View Article] [PubMed]
     [Google Scholar]
  3. Jung S-Y, Lee S-Y, Oh T-K, Yoon J-H. Agromyces allii sp. nov., isolated from the rhizosphere of Allium victorialis var. platyphyllum. Int J Syst Evol Microbiol 2007; 57:588–593 [View Article] [PubMed]
     [Google Scholar]
  4. Heo J, Hamada M, Tamura T, Saito S, Lee SD et al. Agromyces protaetiae sp. nov., isolated from gut of larva of Protaetia brevitarsis seulensis. Int J Syst Evol Microbiol 2020; 70:1259–1265 [View Article]
     [Google Scholar]
  5. Park E-J, Kim M-S, Jung M-J, Roh SW, Chang H-W et al. Agromyces atrinae sp. nov., isolated from fermented seafood. Int J Syst Evol Microbiol 2010; 60:1056–1059 [View Article]
     [Google Scholar]
  6. Guzman J, Ortúzar M, Poehlein A, Daniel R, Trujillo ME et al. Agromyces archimandritae sp. nov., isolated from the cockroach Archimandrita tessellata. Int J Syst Evol Microbiol 2022; 72:5283 [View Article]
     [Google Scholar]
  7. Chen Z, Guan Y, Wang J, Li J. Agromyces binzhouensis sp. nov., an actinobacterium isolated from a coastal wetland of the Yellow River Delta. Int J Syst Evol Microbiol 2016; 66:2278–2283 [View Article]
     [Google Scholar]
  8. Zgurskaya HI, Evtushenko LI, Akimov VN, Voyevoda HV, Dobrovolskaya TG et al. Emended description of the genus Agromyces and description of Agromyces cerinus subsp. cerinus sp. nov., subsp. nov., Agromyces cerinus subsp. nitratus sp. nov., subsp. nov., Agromyces fucosus subsp. fucosus sp. nov., subsp. nov., and Agromyces fucosus subsp. hippuratus sp. nov., subsp. nov. Int J Syst Bacteriol 1992; 42:635–641 [View Article]
     [Google Scholar]
  9. Takeuchi M, Hatano K. Agromyces luteolus sp. nov., Agromyces rhizospherae sp. nov. and Agromyces bracchium sp. nov., from the mangrove rhizosphere. Int J Syst Evol Microbiol 2001; 51:1529–1537 [View Article]
     [Google Scholar]
  10. Hamada M, Saitou S, Tamura T. Agromyces mangrovi sp. nov., a novel actinobacterium isolated from the rhizosphere of a mangrove. Arch Microbiol 2018; 200:939–943 [View Article] [PubMed]
     [Google Scholar]
  11. Jurado V, Groth I, Gonzalez JM, Laiz L, Saiz-Jimenez C. Agromyces subbeticus sp. nov., isolated from a cave in southern Spain. Int J Syst Evol Microbiol 2005; 55:1897–1901 [View Article]
     [Google Scholar]
  12. Hamada M, Shibata C, Tamura T, Suzuki K. Agromyces marinus sp. nov., a novel actinobacterium isolated from sea sediment. J Antibiot 2014; 67:703–706 [View Article] [PubMed]
     [Google Scholar]
  13. Jurado V, Groth I, Gonzalez JM, Laiz L, Schuetze B et al. Agromyces italicus sp. nov., Agromyces humatus sp. nov. and Agromyces lapidis sp. nov., isolated from Roman catacombs. Int J Syst Evol Microbiol 2005; 55:871–875 [View Article] [PubMed]
     [Google Scholar]
  14. Sridhar S, Wang AYM, Chan JFW, Yip CCY, Lau SKP et al. First report of human infection by Agromyces mediolanus, a Gram-positive organism found in soil. J Clin Microbiol 2015; 53:3377–3379 [View Article] [PubMed]
     [Google Scholar]
  15. Lee SA, Heo J, Kim MA, Tamura T, Saitou S et al. Protaetiibacter larvae sp. nov. and Agromyces intestinalis sp. nov., isolated from the gut of larvae of Protaetia brevitarsis seulensis, reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb. nov. and emended description of the genus Pseudolysinimonas. Int J Syst Evol Microbiol 2021; 71: [View Article] [PubMed]
     [Google Scholar]
  16. Tang C, Yang D, Liao H, Sun H, Liu C et al. Edible insects as a food source: a review. Food Prod Process and Nutr 2019; 1:8 [View Article]
     [Google Scholar]
  17. Nikkhah A, Van Haute S, Jovanovic V, Jung H, Dewulf J et al. Life cycle assessment of edible insects (Protaetia brevitarsis seulensis larvae) as a future protein and fat source. Sci Rep 2021; 11:17664 [View Article] [PubMed]
     [Google Scholar]
  18. Lane D. 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics Wiley; 1991
     [Google Scholar]
  19. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article]
     [Google Scholar]
  20. Kumar S, Stecher G, Li M, Knyaz C, Tamura K et al. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol Biol Evol 2018; 35:1547–1549 [View Article]
     [Google Scholar]
  21. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article] [PubMed]
     [Google Scholar]
  22. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article] [PubMed]
     [Google Scholar]
  23. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Biol 1971; 20:406–416 [View Article]
     [Google Scholar]
  24. Manni M, Berkeley MR, Seppey M, Simão FA, Zdobnov EM. BUSCO update: novel and streamlined workflows along with broader and deeper phylogenetic coverage for scoring of eukaryotic, prokaryotic, and viral genomes. Mol Biol Evol 2021; 38:4647–4654 [View Article] [PubMed]
     [Google Scholar]
  25. Tatusova T, DiCuccio M, Badretdin A, Chetvernin V, Nawrocki EP et al. NCBI prokaryotic genome annotation pipeline. Nucleic Acids Res 2016; 44:6614–6624 [View Article] [PubMed]
     [Google Scholar]
  26. Na S-I, Kim YO, Yoon S-H, Ha S-M, Baek I et al. UBCG: up-to-date bacterial core gene set and pipeline for phylogenomic tree reconstruction. J Microbiol 2018; 56:280–285 [View Article] [PubMed]
     [Google Scholar]
  27. Lee I, Ouk Kim Y, Park S-C, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016; 66:1100–1103 [View Article] [PubMed]
     [Google Scholar]
  28. Auch AF, von Jan M, Klenk H-P, Göker M. Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2010; 2:117–134 [View Article]
     [Google Scholar]
  29. Overbeek R, Olson R, Pusch GD, Olsen GJ, Davis JJ et al. The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST). Nucl Acids Res 2014; 42:D206–D214 [View Article]
     [Google Scholar]
  30. Chaudhari NM, Gupta VK, Dutta C. BPGA- an ultra-fast pan-genome analysis pipeline. Sci Rep 2016; 6:24373 [View Article] [PubMed]
     [Google Scholar]
  31. Konstantinidis KT, Tiedje JM. Towards a genome-based taxonomy for prokaryotes. J Bacteriol 2005; 187:6258–6264 [View Article] [PubMed]
     [Google Scholar]
  32. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461–466 [View Article] [PubMed]
     [Google Scholar]
  33. Alcock BP, Raphenya AR, Lau TTY, Tsang KK, Bouchard M et al. CARD 2020: antibiotic resistome surveillance with the comprehensive antibiotic resistance database. Nucleic Acids Res 2020; 48:D517–D525 [View Article] [PubMed]
     [Google Scholar]
  34. McAleese F, Petersen P, Ruzin A, Dunman PM, Murphy E et al. A novel MATE family efflux pump contributes to the reduced susceptibility of laboratory-derived Staphylococcus aureus mutants to tigecycline. Antimicrob Agents Chemother 2005; 49:1865–1871 [View Article]
     [Google Scholar]
  35. Smibert R. Phenotypic characterization. In Methods for General and Molecular Bacteriology American Society for Microbiology; 1994
     [Google Scholar]
  36. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. In MIDI Technical Note vol 101 Newark, DE: MIDI inc; 1990
     [Google Scholar]
  37. Da Costa MS, Albuquerque L, Nobre MF, Wait R. The identification of polar lipids in prokaryotes. In Methods in Microbiology Elsevier; 2011 pp 165–181
     [Google Scholar]
  38. Hamada M, Yamamura H, Komukai C, Tamura T, Suzuki K et al. Luteimicrobium album sp. nov., a novel actinobacterium isolated from a lichen collected in Japan, and emended description of the genus Luteimicrobium. J Antibiot 2012; 65:427–431 [View Article]
     [Google Scholar]
  39. Schleifer KH, Kandler O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 1972; 36:407–477 [View Article] [PubMed]
     [Google Scholar]
  40. Hamada M, Shibata C, Ishida Y, Tamura T, Yamamura H et al. Agromyces iriomotensis sp. nov. and Agromyces subtropicus sp. nov., isolated from soil. Int J Syst Evol Microbiol 2014; 64:833–838 [View Article]
     [Google Scholar]
  41. Rivas R, Trujillo ME, Mateos PF, Martínez-Molina E, Velázquez E. Agromyces ulmi sp. nov., a xylanolytic bacterium isolated from Ulmus nigra in Spain. Int J Syst Evol Microbiol 2004; 54:1987–1990 [View Article]
     [Google Scholar]
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Agromyces larvae sp. nov., a novel actinobacterium isolated from gut of larvae of Protaetia brevitarsis seulensis
Int J Syst Evol Microbiol 73, 005949 (2023); https://doi.org/10.1099/ijsem.0.005949
/content/journal/ijsem/10.1099/ijsem.0.005949
/content/journal/ijsem/10.1099/ijsem.0.005949
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