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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 6

sp. nov., a novel species of obtained from Muscovy ducks () and reclassification of as heterotypic synonym of No Access

Abstract

During a screening study for in two unrelated flocks of Muscovy ducks pharyngeal and cloacal swabs were collected. A total of 59 -like isolates sharing the same colony morphology were subcultured and subsequently characterized. Colonies on bovine blood agar were nonhaemolytic, regular, circular, slightly raised, shiny, intransparent with an entire margin, greyish and had an unguent-like consistency. Isolate AT1 was characterized by 16S rRNA gene sequencing and showed the highest similarity of 96.1 % to the type strain of and 96.0 % to the type strain of , respectively. In addition, and gene sequences also showed the highest similarity to the genus . The phylogenetic comparison of concatenated conserved protein sequences also showed a unique position of AT1 compared to other species of . Full phenotypic characterization of the isolates showed that between two () and 10 () phenotypic characteristics separate the taxon isolated from Muscovy ducks from the accepted species of . Whole genomic sequences of two strains analysed by the type strain genome server showed the highest similarity of 24.9 % to the genome of the type strain of and 23.0 % to the genome of the type strain of . The species sp. nov. is proposed based on the phenotypic and genotypic similarity to as well as differences to the other validly published species of the genus. The leukotoxin protein was not predicted in the genome of AT1. The G+C content of the type strain of sp. nov., AT1 (=CCUG 76754=DSM 115341) is 37.99 mol%, calculated from the whole genome. The investigation further proposes that is reclassified as a later heterotypic synonym of , since and are closely genetically related, and was validly published before .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): clonal relationship between two strains representing two flocks of Muscovy ducks , Danish flocks of Muscovy ducks and pharyngeal/cloacal mucosa of apparently healthy ducks
Funding
This study was supported by the:
  • Statens Jordbrugs- og Veterinærvidenskabelige Forskningsråd
    • Principle Award Recipient: NotApplicable
© 2023 The Authors
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2023-06-26
2025-01-24
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Mannheimia cairinae sp. nov., a novel species of Mannheimia obtained from Muscovy ducks (Cairina moschata) and reclassification of Mannheimia ovis as heterotypic synonym of Mannheimia pernigra
Int J Syst Evol Microbiol 73, 005947 (2023); https://doi.org/10.1099/ijsem.0.005947
/content/journal/ijsem/10.1099/ijsem.0.005947
/content/journal/ijsem/10.1099/ijsem.0.005947
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