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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 6

sp. nov. isolated from the rhizosphere of eggplant in Japan No Access

Abstract

The search for bacteria that can be used as biocontrol agents to control crop diseases yielded a promising candidate, Sm006, which was isolated from the rhizosphere of eggplant () growing in a field in Aichi Prefecture, Japan, in 2006. The cells were Gram-stain-negative, aerobic, non-spore-forming, rod-shaped and motile with one polar flagellum. The results of homology searches and phylogenetic analyses based on the 16S rRNA gene sequence indicated that Sm006 represents a member of the genus . The genomic DNA G+C content was 66.3 mol% and the major cellular fatty acids (more than 5 % of the total fatty acids) were summed feature 8 (Cω7 and/or Cω6), summed feature 3 (Cω7 and/or Cω6), C and C. Phylogenetic analyses using the gene sequence and phylogenomic analysis of the whole genome sequence revealed that Sm006 represents a member of the group; however, its phylogenetic position does not match that of any known species of the genus . The average nucleotide identity and digital DNA–DNA hybridisation values between the strain and closely related species were lower than the thresholds for prokaryotic species delineation (95–96 and 70 %, respectively), with the highest values observed for TUM18999 (92.05 and 46.3 %, respectively). Phenotypic characteristics, cellular fatty acid composition and possession of 2,4-diacetylphloroglucinol biosynthetic gene cluster could be used to differentiate the strain from its closest relatives. The phenotypic, chemotaxonomic and genotypic data obtained during this study indicated that Sm006 represents a novel species of the genus , for which we propose the name sp. nov., with Sm006 (= MAFF 212523 = ICMP 24689) as the type strain.

  • Received:
  • Accepted:
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Keyword(s): biocontrol , eggplant , phylogenomic analysis , polyphasic taxonomy , Pseudomonas resinovorans group and Solanum melongena
© 2023 The Authors
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2023-06-22
2024-12-05
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Pseudomonas solani sp. nov. isolated from the rhizosphere of eggplant in Japan
Int J Syst Evol Microbiol 73, 005942 (2023); https://doi.org/10.1099/ijsem.0.005942
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