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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 7

sp. nov., isolated from a till sample of Collins glacier front, Antarctica No Access

Abstract

A pink-coloured, Gram-stain-positive, aerobic and rod-shaped strain, designated 75, was isolated from Collins glacier front till, Antarctica. Strain 75 was non-motile and non-spore-forming. Growth was observed at pH 6.0–9.0 (optimum, pH 7.0), at 4–45 °C (optimum, 20 °C) and with 0–9 % (w/v) NaCl (optimum, 1%). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain 75 belongs to the genus , and is related to DSM 104003, KCTC29240 and KCTC 39118 (96.1, 96.0 and 95.7 % sequence similarity, respectively). The main polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and a phosphoglycolipid. The major cellular fatty acids were identified as C, iso- C, 10-methyl C and C 8. MK-7 and MK-8(H) were found to be the dominant menaquinones. Whole-cell hydrolysates contained -diaminopimelic acid, ribose, galactose, glucose and rhamnose. The genome of strain 75 is 3.82 Mb long in size with a G+C content of 73.1 mol%. On the basis of phenotypic, molecular and chemotaxonomic characteristics, strain 75 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 75 (=CCTCC AA 2019032=KCTC 49334).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Antarctica , glacier , Rhodococcus antarcticus and till
Funding
This study was supported by the:
  • Chinese Polar Environment Comprehensive Investigation and Assessment Programmes (Award IC201706)
    • Principle Award Recipient: FangPeng
  • Ministry of Science and Technology of the People's Republic of China (Award NIMR-2022-8)
    • Principle Award Recipient: FangPeng
  • National Science and Technology Program during the Twelfth Five-year Plan Period (Award 2021FY100902)
    • Principle Award Recipient: FangPeng
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 42076230)
    • Principle Award Recipient: FangPeng
© 2023 The Authors
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2024-12-05
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Rhodococcus antarcticus sp. nov., isolated from a till sample of Collins glacier front, Antarctica
Int J Syst Evol Microbiol 73, 005940 (2023); https://doi.org/10.1099/ijsem.0.005940
/content/journal/ijsem/10.1099/ijsem.0.005940
/content/journal/ijsem/10.1099/ijsem.0.005940
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