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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 5

sp. nov., isolated from tomato rhizosphere soil No Access

Abstract

Two aerobic, Gram-stain-negative, non-motile and non-spore-forming rods bacterial strains, designated MMSM20 and MMSM24, were isolated from tomato rhizosphere soil and could produce indole-3-acetic acid and siderophore. Phylogenetic analyses based on 16S rRNA gene sequences and 92 core genes showed that strains MMSM20 and MMSM24 belonged to the genus and were most closely related to three validly published species G39, DSM 17494 and DSM 21029. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strains MMSM20 and MMSM24 were 97.6 and 81.0 %, respectively, demonstrating that they were conspecific. The ANI and dDDH values between the two strains and the three type strains above were below the threshold values for species delimitation. The genomic DNA G+C contents of strains MMSM20 and MMSM24 were 66.6 and 66.4 mol%, respectively. The major fatty acids of the two strains were identified as C 2OH, summed feature 8 (C 7 and/or C 6); the predominant quinone was ubiquinone 10; the polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, sphingoglycolipid and unidentified lipids. Results of phenotypic and genotypic analyses supported that strains MMSM20 and MMSM24 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MMSM20 (=GDMCC 1.3401=JCM 35647).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): novel species , polyphasic taxonomy , Sphingomonas and tomato rhizosphere soil
Funding
This study was supported by the:
  • GDAS’ Project of Science and Technology Development (Award 2021GDASYL-2021010309)
    • Principle Award Recipient: HonghongDong
  • Guangdong Special Support Plan (Award 2021JC06N628)
    • Principle Award Recipient: HonghuiZhu
  • The Guangdong Strategic Special Fund for Rural Revitalization (Award 2022-440000-43010104-9463)
    • Principle Award Recipient: HonghuiZhu
  • National Natural Science Foundation of China (Award 32102276)
    • Principle Award Recipient: HonghongDong
© 2023 The Authors
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2023-05-31
2024-12-03
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Sphingomonas lycopersici sp. nov., isolated from tomato rhizosphere soil
Int J Syst Evol Microbiol 73, 005920 (2023); https://doi.org/10.1099/ijsem.0.005920
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