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Volume 73, Issue 5

sp. nov., isolated from soil of a mangrove nature reserve No Access

Abstract

A Gram-stain-negative, non-motile, aerobic and ovoid-rod-shaped bacterial strain, designated as 2C3345, was isolated from soil of a mangrove nature reserve in Shenzhen, PR China and its taxonomic position was investigated. The 16S rRNA gene sequence of strain 2C3345 exhibited the highest similarity value to that of the type strains NBRC 113114 (98.4 %), KCTC 42013 (98.1 %), DSM 15422 (98.0 %) and DSM 13605 (97.9 %). Strain 2C3345 grew optimally at 28–30 °C, pH 7.0 and without NaCl. The predominant ubiquinone in 2C3345 was identified as Q-8. The major fatty acids of 2C3345 were iso-C, iso-C and summed feature 9 (is0-Cω9 and/or 10-methyl C). The major polar lipids of 2C3345 were phosphatidylcholine (PC), phosphatidylglycerol (PG), diphosphatidglycerol (DPG), phosphatidylethanolamine (PE), an unidentified phospholipid, an unidentified aminolipid and two unidentified lipids. The DNA G+C content of strain 2C3345 was 69.9 %. The draft genome sequence of 2C3345 included 2 888 645 bp in total (=310 730 bp) with a medium read coverage of 388.6×and 21 scaffolds. DNA–DNA hybridisation with type strains of species of the genus showed 24.4, 22.4, 24.1 and 23.2% relatedness to NBRC 113114, KCTC 42013, DSM 15422 and DSM 13605, respectively. And the average nucleotide identity by (ANIb) values between 2C3345 and these four reference strains were 81.9, 80.0, 81.2 and 80.6 %, respectively, lower than the 95–96% threshold value for dividing prokaryotic species. On the basis of its phenotypic, chemotaxonomic, phylogenetic and phylogenomic properties, strain 2C3345 is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 2C3345 (=KCTC 92730 =MCCC 1K08335).

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Keyword(s): mangrove nature reserve , soil and Thermomonas mangrovi sp. nov
Funding
This study was supported by the:
  • Shenzhen Environmental Science and New Energy Technology Engineering Laboratory (Award JCYJ20210324093409025)
    • Principle Award Recipient: YingXu
© 2023 The Authors
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2024-12-10
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Thermomonas mangrovi sp. nov., isolated from soil of a mangrove nature reserve
Int J Syst Evol Microbiol 73, 005915 (2023); https://doi.org/10.1099/ijsem.0.005915
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