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Volume 73, Issue 5

sp. nov., isolated from Stegodon Sea Cave in Thailand No Access

Abstract

A novel Gram-stain-negative, obligately aerobic, short rod-shaped and motile bacterium, designated strain BC00092, was isolated from brackish ground water collected in Stegodon Sea Cave located at Satun UNESCO Global Geopark, Satun Province, Thailand. The phylogenetic analysis of BC00092 based on 16S rRNA gene sequences revealed that the strain represented a member of the genus and was closely related to DSM 17879 (96.68 %) and IMCC25680 (94.89 %). The average nucleotide identity and digital DNA–DNA hybridization values calculated from the whole-genome sequences between BC00092 and closely related type strains of species within the family were lower than the species demarcation threshold values of 95 and 70 %, respectively. Moreover, five conserved signature indels of members of the family were found in the protein sequences from the annotated assembled genome of BC00092. According to the results of the polyphasic taxonomic study, strain BC00092 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is BC00092 (TBRC 13508 = KCTC 92111).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): cave , Leeia speluncae sp. nov and Thailand
Funding
This study was supported by the:
  • Korea Research Institute of Bioscience and Biotechnology
    • Principle Award Recipient: Jung-SookLee
  • National Center for Genetic Engineering and Biotechnology
    • Principle Award Recipient: PattarapornYukphan
  • RDI Management for National Strategic and Network Division
    • Principle Award Recipient: PattarapornYukphan
© 2023 The Authors
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2023-05-30
2024-12-06
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Leeia speluncae sp. nov., isolated from Stegodon Sea Cave in Thailand
Int J Syst Evol Microbiol 73, 005908 (2023); https://doi.org/10.1099/ijsem.0.005908
/content/journal/ijsem/10.1099/ijsem.0.005908
/content/journal/ijsem/10.1099/ijsem.0.005908
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