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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 5

gen. nov., sp. nov., a novel bacterium isolated from phycosphere No Access

Abstract

A novel strain of a member of the family was isolated from the phycosphere of a diatom and designated as LMIT007. LMIT007 could form milk-white, opaque, circular and smooth colonies on 2216E marine agar. LMIT007 cells were around 1.0–1.8 µm long, 0.8–1.8 µm wide, round or oval shaped and had polar flagella but were non-motile. Optimum conditions for growth were 25 °C, pH 7.0 and 6 % (w/v) NaCl. The results of 16S rRNA gene-based analysis indicated that LMIT007 had the highest similarity with the type strains JC2043 (95.95 %), JW12 (95.60 %) and KCTC 22164 (94.21 %). Furthermore, the results of phylogenetic analysis based on 16S rRNA gene sequences and of phylogenomic analysis indicated that LMIT007 could be clustered into the family but formed a separate branch. The genome size of the strain was 2.95 Mbp and the DNA G+C content was 41.6 %. The average nucleotide identity (ANI) values of orthologous genes between LMIT007 and species of other closely related genera within the family ranged from 66.9 to 69.2 %, and the average amino acid identity (AAI) values ranged from 60.0 to 65.7 %. The main respiratory quinone was ubiquinone-8. The major fatty acids were summed feature 3 (Cω7 / Cω6) and C. The polar lipid profile contain phosphatidylethanolamine, phosphatidylglycerol, aminolipid, two phospholipid and an unknown polar lipid. On the basis of the results of the polyphasic analysis, strain LMIT007 is suggested to represent a novel genus and species within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is LMIT007 (=MCCC 1K08161=KCTC 92597).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genome reduction , Opacimonas , phycosphere and polyphasic analysis
Funding
This study was supported by the:
  • Scientific Research Foundation of Shantou University (Award NTF21030)
    • Principle Award Recipient: GuanjingCai
  • Guangdong Science and Technology Department (Award 2022B1515020017)
    • Principle Award Recipient: HuiWang
  • National Natural Science Foundation of China (Award 32070113)
    • Principle Award Recipient: HuiWang
  • National Natural Science Foundation of China (Award 92051118)
    • Principle Award Recipient: HuiWang
© 2023 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005904
2023-05-26
2025-01-14
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/content/journal/ijsem/10.1099/ijsem.0.005904
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Opacimonas viscosa gen. nov., sp. nov., a novel bacterium isolated from phycosphere
Int J Syst Evol Microbiol 73, 005904 (2023); https://doi.org/10.1099/ijsem.0.005904
/content/journal/ijsem/10.1099/ijsem.0.005904
/content/journal/ijsem/10.1099/ijsem.0.005904
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