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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 5

sp. nov., isolated from seawater of the Tangyin hydrothermal field in the Okinawa Trough No Access

Abstract

A Gram-stain-negative, strictly aerobic, rod-shaped and non-flagellated marine bacterium, designated strain LXI357, was isolated from deep-sea water sampled at the Tangyin hydrothermal field in the Okinawa Trough. The growth temperature range was 20–45 °C (optimum, 28 °C). Strain LXI357 was also able to grow at pH 5.0–7.5 (optimum, pH 6.0–7.0) and in the presence of 0.5–11 % (optimum, 7%, w/v) NaCl. Strain LXI357 was oxidase-negative and catalase-positive. The predominant fatty acids were C 7 and C. The major polar lipids of strain LXI357 contained phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phospholipid, sphingoglycolipid, diphosphatidylglycero and an unidentified aminolipid. Based on the results of 16S rRNA gene sequence analysis, strain LXI357 belonged to the genus and was most closely related to CBK3Z-3 (96.28%, 16S rRNA gene sequence similarity), followed by Yeonmyeong 1-13 (95.67%), JLT832 (95.46%) and YC7378 (95.43%). Genome relatedness between strain LXI357 and CBK3Z-3 was computed using average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity, with values of 76.02, 20.9 and 71.1 %, respectively. The genomic DNA G+C content of strain LXI357 is 64.1 mol%. In addition, strain LXI357 has multiple genes related to sulphur metabolism, including genes encoding for the Sox system. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished strain LXI357 from its closest phylogenetic neighbours. According to the results of polyphasic analyses, strain LXI357 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LXI357 (=MCCC 1K06076=KCTC 82726).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): hydrothermal field , Okinawa Trough and Stakelama marina
Funding
This study was supported by the:
  • Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology (Award 2016ASKJ14)
    • Principle Award Recipient: Xiao-HuaZhang
  • Major State Basic Research Development Program of China (Award 2013CB429700)
    • Principle Award Recipient: MinYu
  • National Key R&D Program of China (Award 2018YFC0310701)
    • Principle Award Recipient: MinYu
  • National Natural Science Foundation of China (Award 41976137)
    • Principle Award Recipient: MinYu
  • National Natural Science Foundation of China-Shandong Joint Fund (Award U1706208)
    • Principle Award Recipient: MinYu
© 2023 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005902
2023-05-23
2025-01-24
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Stakelama marina sp. nov., isolated from seawater of the Tangyin hydrothermal field in the Okinawa Trough
Int J Syst Evol Microbiol 73, 005902 (2023); https://doi.org/10.1099/ijsem.0.005902
/content/journal/ijsem/10.1099/ijsem.0.005902
/content/journal/ijsem/10.1099/ijsem.0.005902
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