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Volume 73, Issue 5

gen. nov., sp. nov., isolated from a hot spring No Access

Abstract

An isolate, designated CFH 74404, was recovered from a hot spring in Tengchong, Yunnan province, PR China. Phylogenetic analysis indicated that the isolate belongs to the family and showed the highest 16S rRNA gene sequence similarity to KI4 (93.6 %), WKT50.2 (93.1 %), DSM 5159 (92.0 %) and KI3 (91.7 %). The average amino acid identity and average nucleotide identity values between strain CFH 74404 and the closest relatives were 42.0–75.9 % and 67.0–77.3 %, respectively. Cells of strain CFH 74404 stained Gram-positive and were aerobic, non-motile and short rod-shaped. Growth occurred at 20–65 °C (optimum, 55 °C), pH 6.0–8.0 (optimum, pH 7.0) and with up to 2.0 % (w/v) NaCl (optimum 0–1.0 %, w/v). The predominant respiratory quinone was MK-8. The major fatty acids (>10 %) were C (50.8 %) and C (16.8 %). The polar lipid profile of strain CFH 74404 included diphosphatidylglycerol, four unidentified phosphoglycolipids, phosphatidylinositol and three unidentified glycolipids. The G+C content of the genomic DNA was determined to be 67.1 mol% based on the draft genome sequence. On the basis of phenotypic, phylogenetic and genotypic analyses, it is concluded that strain CFH 74404 represents a new species of a novel genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is CFH 74404 (=KCTC 62930=CGMCC 1.61585).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): hot spring , Thermalbibacter gen. nov. , Thermalbibacter longus sp. nov. , Thermomicrobiaceae and Thermomicrobiota
Funding
This study was supported by the:
  • Doctor Scientific Research Fund of Xinxiang Medical University (Award XYBSKYZZ201625)
    • Principle Award Recipient: HongMing
  • Key Technologies R&D Program of Henan Province (Award 202102110107)
    • Principle Award Recipient: HongMing
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2021FY100900)
    • Principle Award Recipient: Jian-YuJiao
  • National Natural Science Foundation of China (Award 91951205 and 92251302)
    • Principle Award Recipient: Wen-JunLi
© 2023 Sun Yat-Sen University
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Thermalbibacter longus gen. nov., sp. nov., isolated from a hot spring
Int J Syst Evol Microbiol 73, 005891 (2023); https://doi.org/10.1099/ijsem.0.005891
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