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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 4

Comparative genomic analysis of the genus and taxonomic study of sp. nov., isolated from Chinese rice wine starter No Access

Abstract

In this study, three lactic acid bacteria, namely, HBUAS51963, HBUAS51964 and HBUAS51965, were isolated from Chinese rice wine starter sampled in Fangxian County, PR China. All were non-motile, non-spore-forming and Gram-positive spherical cells. Their taxonomic status was characterized using a polyphasic approach. Genome-based analysis revealed that all three strains were phylogenomically related to KCTC 3751 and ATCC 33313. The digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between the three strains and the phylogenetically related type strains were less than 54.8 and 93.8 %, respectively, and thus, they were below the thresholds of dDDH and ANI for species definition. The genomic DNA G+C content was 38.6 mol %. The predominant fatty acid methyl esters (>10 %) were C, C cyc11 and summed feature 10 (C cyc11 and/or ECL 17.834). The polar lipids in the cells of strain HBUAS51963 were mainly phosphatidylglycerol, diphosphatidylglycerol, unidentified glycolipids, phospholipids and lipids. Finally, the three strains were capable of producing -lactic acid (4.29 g l) and various organic acids such as tartaric, acetic, lactic and succinic acids. Overall, the results of genotypic, phenotypic and genomic analyses suggest that the three strains represent a new species of the genus , for which the name sp. nov. is proposed. The type strain is HBUAS51963 (=GDMCC 1.3506= JCM 35803).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genome , rice wine starter , taxonomy and Weissella
Funding
This study was supported by the:
  • Hubei University of Arts and Science Cultivation Fund for Teachers' Scientific Research Ability: Technological Innovation Team (Award 2020kypytd009)
    • Principle Award Recipient: ZhuangGuo
  • Hubei Provincial Natural Science Foundation Joint Fund for Innovation and Development Project (Award 2022CFD008)
    • Principle Award Recipient: ZhuangGuo
© 2023 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005870
2023-04-27
2025-01-17
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Comparative genomic analysis of the genus Weissella and taxonomic study of Weissella fangxianensis sp. nov., isolated from Chinese rice wine starter
Int J Syst Evol Microbiol 73, 005870 (2023); https://doi.org/10.1099/ijsem.0.005870
/content/journal/ijsem/10.1099/ijsem.0.005870
/content/journal/ijsem/10.1099/ijsem.0.005870
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