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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 5

gen. nov., sp. nov., a novel member of the family isolated from a coastal shrimp pond in Guangxi, PR China No Access

Abstract

A Gram-stain-negative, strictly aerobic, rod-shaped and motile bacterium with bipolar flagella, designated G-43, was isolated from a surface seawater sample collected from an aquaculture in Guangxi, PR China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain G-43 was most closely related to the family and distantly to the most closely related genera and (95.52 % and 94.45–94.76 % 16S rRNA gene sequence similarity, respectively), while similarity values to other type strains were lower than 94.0 %. Strain G-43 was found to grow at 4–30 °C (optimum, 25–28 °C), pH 6–9.0 (optimum, pH 7.0) and with 0–4.0 % NaCl (w/v; optimum at 2 % NaCl). Chemotaxonomic analysis of strain G-43 indicated that the sole respiratory quinone was ubiquinone-8, the predominant cellular fatty acids were C, summed feature 3 (C ω7 and/or C ω6) and summed feature 8 (C ω7 and/or C ω6), and the major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, aminolipid, diphosphatidylglycerol, phospholipids and an unidentified lipid. The G+C content of the genomic DNA was 55.4 mol%. The phylogenetic, genotypic, phenotypic and chemotaxonomic data demonstrate that strain G-43 represents a novel species in a novel genus within the family , for which the name gen. nov., sp. nov. is proposed. Strain G-43 (=KCTC 72750= CCTCC AB 2022321) is the type and only strain of .

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  • Accepted:
  • Published Online:
Keyword(s): aquaculture , halophilic , novel genus and Parathalassolituus penaei
Funding
This study was supported by the:
  • Special Fund for Scientific Innovation Strategy-Construction of High-level Academy of Agriculture Science (Award XDA23040401)
    • Principle Award Recipient: DongruQiu
  • National Natural Science Foundation of China (Award 32102769)
    • Principle Award Recipient: JingchengDai
  • National Key R&D Program of China (Award 2019YFA0905500)
    • Principle Award Recipient: DongruQiu
© 2023 The Authors
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2023-05-15
2025-01-16
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Parathalassolituus penaei gen. nov., sp. nov., a novel member of the family Oceanospirillaceae isolated from a coastal shrimp pond in Guangxi, PR China
Int J Syst Evol Microbiol 73, 005867 (2023); https://doi.org/10.1099/ijsem.0.005867
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/content/journal/ijsem/10.1099/ijsem.0.005867
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