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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 5

sp. nov., isolated from deep-sea sediment of the Indian Ocean No Access

Abstract

A taxonomic study was carried out on strain GC03-9, which was isolated from deep-sea sediment of the Indian Ocean. The bacterium was Gram-stain-negative, catalase-positive, oxidase-negative, rod-shaped and gliding motile. Growth was observed at salinities of 0–9 % and at temperatures of 10–42 °C. The isolate could degrade gelatin and aesculin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GC03-9 belonged to the genus , with the highest sequence similarity to JCM 33424 (97.9 %), followed by KCTC 23123 (97.2 %) and other species of the genus (93.4–96.3 %). The average nucleotide identity and the digital DNA–DNA hybridization estimate values between strain GC03-9 and JCM 33424 and KCTC 23123 were 25.1 and 18.7 % and 82.47 and 75.69 %, respectively. The principal fatty acids were iso-C (28.0 %), iso-C 3OH (13.4 %), summed feature 9 (iso-C 9 and/or 10-methyl C; 13.3 %) and summed feature 3 (C 7 and/or C 6; 11.0 %). The G+C content of the chromosomal DNA was 41.17 mol%. The respiratory quinone was determined to be menaquinone-6 (100 %). Phosphatidylethanolamine, one unknown phospholipid, three unknown aminolipids and two unknown polar lipids were present. The combined genotypic and phenotypic data showed that strain GC03-9 represents a novel species within the genus , for which the name sp. nov. is proposed, with the type strain GC03-9 (=MCCC M25440=KCTC 92235).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): deep-sea sediment , Gramella oceanisediminis sp. nov. , Indian Ocean and taxonomy
Funding
This study was supported by the:
  • Open Fund of Key Laboratory of Biotechnology and Bioresources Utilization (Dalian Minzu University), Ministry of Education China (Award No. KF202006)
    • Principle Award Recipient: NotApplicable
  • the National Natural Science Foundation of China (Award 42276140)
    • Principle Award Recipient: NotApplicable
  • Deep Sea Biological Resources Program (Award No. DY135-B2-11)
    • Principle Award Recipient: NotApplicable
  • Open access funding provided by the National Key Research and Development Program of China (Award No. 2018YFC0310701)
    • Principle Award Recipient: NotApplicable
© 2023 The Authors
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Gramella oceanisediminis sp. nov., isolated from deep-sea sediment of the Indian Ocean
Int J Syst Evol Microbiol 73, 005861 (2023); https://doi.org/10.1099/ijsem.0.005861
/content/journal/ijsem/10.1099/ijsem.0.005861
/content/journal/ijsem/10.1099/ijsem.0.005861
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