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Volume 73, Issue 5

sp. nov., isolated from freshwater wetland sediment No Access

Abstract

A Gram-stain-positive, motile, rod-shaped, facultatively anaerobic bacterium, designated strain WST5, isolated from sediment was characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain WST5 was most closely related to CJ25 (96.8 % similarity). The genome size of the WST5 was 6.5 Mb, contained 4500 predicted protein-coding genes, and had a DNA G+C content of 46.6%. The values of whole-genome average nucleotide identity analysis and digital DNA–DNA hybridization between strain WST5 and its closely related type strains were less than 76 and 25.6 %, respectively. The predominant cellular fatty acids (>10 %) were anteiso-C and C ω5 and the main menaquinone was MK-7. The major polar lipids were identified as diphospholidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unknown aminophospholipids. Based on the results of phenotypic, genotypic, chemotaxonomic and phylogenetic analyses, strain WST5 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WST5 (=NBRC 115194 =CGMCC 1.18706).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Firmicutes , new taxa , Paenibacillus and sediment
Funding
This study was supported by the:
  • Science and Technology Planning Project of Inner Mongolia (Award JH20180633)
    • Principle Award Recipient: ZhihuaBao
  • Natural Science Foundation of Inner Mongolia (Award 2019MS04005)
    • Principle Award Recipient: ZhihuaBao
  • the Science and Technology Major Project on Lakes of Inner Mongolia grant (Award ZDZX2018054)
    • Principle Award Recipient: LixinWang
© 2023 The Authors
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2023-05-12
2024-12-05
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Paenibacillus sedimenti sp. nov., isolated from freshwater wetland sediment
Int J Syst Evol Microbiol 73, 005860 (2023); https://doi.org/10.1099/ijsem.0.005860
/content/journal/ijsem/10.1099/ijsem.0.005860
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