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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 4

sp. nov., sp. nov. and sp. nov., three marine bacteria isolated from a tidal flat No Access

Abstract

Three strains, TT30, TT37 and L3, were isolated from tidal flat samples. Cells were Gram-stain-negative, non-motile and rod shaped. Cells of strains TT30 and TT37 were able to grow in a medium containing 1.0–15.0 % (w/v) NaCl (optimum, 3.0 and 4.0 %, respectively), and cells of strain L3 was able to grow in a medium containing 1.0–10.0 % (w/v) NaCl (optimum, 1.0 %). Growth of the three strains was observed at pH 6.0–10.0 and at 10–40 °C. Strains TT30, TT37 and L3 showed the highest similarity to DSM 11525 (97.7 %), . CGMCC 1.10658 (98.0 %) and DSM 6810 (97.9 %), respectively. Results of phylogenetic analyses indicated that the three isolates represented two distinct lineages within the genus . The DNA G+C contents of strains TT30, TT37 and L3 were 61.3, 60.9 and 60.2%, respectively. The average nucleotide identity and DNA–DNA hybridization values among strains TT30, TT37 and L3 and the reference strains were 84.4–87.4 and 19.6–28.9 %, respectively. Differential phenotypic properties, chemotaxonomic differences, phylogenetic distinctiveness, together with the genomic data, demonstrated that strains TT30, TT37 and L3 represent novel species of the genus , which are named sp. nov. (TT30=KCTC 92167=MCCC 1K07276), sp. nov. (TT37=KCTC 92168=MCCC 1K07277) and sp. nov. (L3=KCTC 92165=MCCC 1K07278).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genomic analysis , Microbulbifer , phylogenetic analysis and tidal flat
Funding
This study was supported by the:
  • the Scientific Research Fund of the Second Institute of Oceanography (Award JZ1901)
    • Principle Award Recipient: Xue-WeiXu
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2021FY100900)
    • Principle Award Recipient: YuehongWu
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2019FY100700)
    • Principle Award Recipient: Xue-WeiXu
© 2023 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005842
2023-04-20
2025-01-20
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Microbulbifer zhoushanensis sp. nov., Microbulbifer sediminum sp. nov. and Microbulbifer guangxiensis sp. nov., three marine bacteria isolated from a tidal flat
Int J Syst Evol Microbiol 73, 005842 (2023); https://doi.org/10.1099/ijsem.0.005842
/content/journal/ijsem/10.1099/ijsem.0.005842
/content/journal/ijsem/10.1099/ijsem.0.005842
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